Maulana Azad Medical College, New Delhi, India.
Maulana Azad Medical College, New Delhi, India;
Allergol Immunopathol (Madr). 2021 Nov 1;49(6):42-47. doi: 10.15586/aei.v49i6.91. eCollection 2021.
Iron deficiency anemia (IDA) is the most prevalent micronutrient deficiency in preschool children in developing countries including India. IDA is associated with immune perturbation, which is reflected in greater frequency of infections in these children. Recent research has shown three distinct monocyte subsets with distinct functions linked to infectious, inflammatory, and autoimmune diseases. These subsets have not been studied in children with IDA.
The aim of the study was to assess the percentage of monocyte population and the three subset populations in children with IDA and to compare the data with age-matched healthy controls.
Venous blood samples (5 mL) from 40 IDA children and 20 controls were collected after taking informed consent. Monocyte subpopulations were compared across the two groups. The outcome variables were calculated using Students Independent t-test or Mann-Whitney U test. P value of <0.05 was taken as significant.
No significant difference was found in the absolute numbers as well as percentages of total monocytes between the control and case (study) group. Children in the IDA group showed a significant (p = 0.03) decrease in the nonclassical subset population when compared to the control group.
This is the first study done on monocyte subsets in iron-deficient children. Decrease in nonclassical monocytes observed may be associated with a pro-inflammatory state and increased risk of inflammatory and auto immune diseases. Follow-up studies are needed to confirm these findings.
缺铁性贫血（IDA）是发展中国家（包括印度）学龄前儿童中最常见的微量营养素缺乏症。IDA 与免疫紊乱有关，这反映在这些儿童感染的频率更高。最近的研究表明，单核细胞有三个不同的亚群，它们具有不同的功能，与感染、炎症和自身免疫性疾病有关。这些亚群在患有 IDA 的儿童中尚未得到研究。
本研究旨在评估 IDA 儿童单核细胞群体及其三个亚群的百分比，并将数据与年龄匹配的健康对照组进行比较。
在获得知情同意后，采集 40 名 IDA 儿童和 20 名对照者的静脉血样（5 毫升）。比较两组间单核细胞亚群。使用学生独立 t 检验或曼-惠特尼 U 检验比较两组间的结果变量。P 值<0.05 被认为具有统计学意义。
对照组和病例组（研究组）之间的单核细胞绝对数和百分比均无显著差异。与对照组相比，IDA 组的非经典亚群显著减少（p=0.03）。
这是第一项关于缺铁儿童单核细胞亚群的研究。观察到的非经典单核细胞减少可能与促炎状态和增加炎症及自身免疫性疾病的风险有关。需要进一步的研究来证实这些发现。
