Xu Bing, Liu Jia, Zhao Chunzhi, Sun Shiqing, Zhao Yongjun, Liu Juan, Xu Jie, Wu Daoji
School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, 250101, People's Republic of China.
Co-innovation center of Green Building, Jinan, 250101, People's Republic of China.
World J Microbiol Biotechnol. 2021 Nov 11;37(12):216. doi: 10.1007/s11274-021-03184-2.
The algae-based technology has a positive effect on the treatment of biogas slurry and the purification of biogas, while vitamin B12 (VB12) is one of the important regulatory substances in the algae-based cultivation system. In this study, different concentrations of VB12 were used in three microalgal treatment technologies to assess their effect on simultaneous removal of nutrients from biogas slurry and removal of CO from raw biogas. Results showed that Chlorella vulgaris exhibited higher growth rate, mean daily productivity, chlorophyll a content, carbonic anhydrase activity and better photosynthetic properties when co-cultivated with Ganoderma lucidum, rather than when co-cultivated with activated sludge or under mono-cultivation. Maximum mean chemical oxygen demand, total nitrogen, total phosphorus and CO removal efficiencies were found to be 84.29 ± 8.28%, 83.27 ± 8.14%, 85.27 ± 8.46% and 65.71 ± 6.35%, respectively when microalgae were co-cultivated with Ganoderma lucidum under 100 ng L of VB12. This study shows the potential of microalgae and fungi co-cultivation supplemented with VB12 for the simultaneous upgradation of biogas production as well as for the purification of biogas slurry.
基于藻类的技术对沼液处理和沼气净化具有积极作用，而维生素B12（VB12）是基于藻类的培养系统中的重要调节物质之一。在本研究中，在三种微藻处理技术中使用了不同浓度的VB12，以评估其对同时去除沼液中营养物质和去除原料沼气中CO的效果。结果表明，与灵芝共培养时，普通小球藻的生长速率、平均日生产力、叶绿素a含量、碳酸酐酶活性更高，光合特性更好，而与活性污泥共培养或单培养时则不然。当微藻在100 ng/L的VB12条件下与灵芝共培养时，化学需氧量、总氮、总磷和CO的最大平均去除效率分别为84.29±8.28%、83.27±8.14%、85.27±8.46%和65.·71±6.35%。本研究表明，补充VB12的微藻与真菌共培养在同时提高沼气产量和净化沼液方面具有潜力。
