Department of Clinical Laboratory, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
Department of Microbiology and Infectious Disease, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143-8540, Japan.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005115.
Strain TUM18999 was isolated from the skin of a patient with burn wounds in Japan. The strain was successfully cultured at 20-42 °C (optimum, 30-35 °C) in 1.0-4.0% NaCl (w/v) and at pH 5.5-9.5, optimum pH 5.5-8.5. The phylogenetic tree reconstructed using 16S rRNA, , and gene sequences indicated that strain TUM18999 is closely related to MCC10330. Although the partial 16S rRNA gene sequence (1412 bp) of TUM18999 exhibits high similarity to those of NBRC 14159 (99.08 %) and MCC10330 (98.51 %), multi-locus sequence analysis using 16S rRNA, , and genes reveals a clear distinction between TUM18999 and other species. In addition, an average nucleotide identity >90 % was not observed in the group. Moreover, TUM18999 and can be distinguished based on the minimum inhibitory concentration for carbapenem. Meanwhile, the cellular fatty acids are enriched with C 7/C 6 (34.35 %), C 7/C 6 (24.22 %), C (19.79 %) and C (8.25 %). Based on this evidence, strain TUM18999 can be defined as representing a novel species, with the proposed name sp. nov. The type strain is TUM18999 (GTC 22698=NCTC 14580).
菌株 TUM18999 从日本一位烧伤患者的皮肤中分离得到。该菌株可在 20-42°C（最佳，30-35°C）、1.0-4.0% NaCl（w/v）和 pH5.5-9.5（最佳 pH5.5-8.5）的条件下成功培养。基于 16S rRNA、、和 基因序列构建的系统发育树表明，菌株 TUM18999 与 MCC10330 密切相关。尽管 TUM18999 的部分 16S rRNA 基因序列（1412bp）与 NBRC 14159（99.08%）和 MCC10330（98.51%）高度相似，但基于 16S rRNA、、和 基因的多位点序列分析显示，TUM18999 与其他种属之间存在明显差异。此外，在 组中未观察到平均核苷酸同一性>90%。此外，基于碳青霉烯类抗生素的最低抑菌浓度可以区分 TUM18999 和 。同时，细胞脂肪酸富含 C7/C6（34.35%）、C7/C6（24.22%）、C19（19.79%）和 C8（8.25%）。基于这些证据，菌株 TUM18999 可被定义为代表一个新的 种，其名称为 sp. nov.。模式菌株为 TUM18999（GTC 22698=NCTC 14580）。
