Maganha de Almeida Kumlien Ana C, Pérez-Vega Clara, González-Villalobos Edgar, Borrego Carles M, Balcázar José Luis
Catalan Institute for Water Research (ICRA), Girona, Spain; University of Girona, Girona, Spain.
Catalan Institute for Water Research (ICRA), Girona, Spain; Group of Molecular Microbial Ecology, Institute of Aquatic Ecology, University of Girona, Girona, Spain.
Virus Res. 2022 Jan 2;307:198623. doi: 10.1016/j.virusres.2021.198623. Epub 2021 Nov 8.
In this study, we present the complete, annotated genome of a new member of the Tequatrovirus (T4-like) genus, Escherichia phage vB_EcoM_C2-3. This phage has an isometric head (92 nm in diameter) and a contractile tail (114 nm in length). Its genome consists of a linear, double-stranded DNA of 167,069bp with an average G+C content of 35.3%. There are 267 predicted genes, of which 125 encode functional proteins, including those for DNA replication, transcription and packaging, phage morphogenesis and cell lysis. Neither genes involved in the regulation of lysogeny nor antibiotic resistance genes were identified. Based on our results, its genomic features provide valuable insights into the use of a potential biocontrol agent, as Escherichia phage vB_EcoM_C2-3 exhibited lytic activity against E. coli, including multidrug-resistant strains.
在本研究中，我们展示了嗜食埃希氏菌噬菌体vB_EcoM_C2-3（T4样）属一个新成员的完整注释基因组。该噬菌体有一个等轴状头部（直径92纳米）和一条收缩尾（长度114纳米）。其基因组由一条167,069bp的线性双链DNA组成，平均G+C含量为35.3%。有267个预测基因，其中125个编码功能蛋白，包括参与DNA复制、转录和包装、噬菌体形态发生及细胞裂解的蛋白。未鉴定出参与溶原性调控的基因和抗生素抗性基因。基于我们的结果，其基因组特征为一种潜在生物防治剂的应用提供了有价值的见解，因为嗜食埃希氏菌噬菌体vB_EcoM_C2-3对大肠杆菌包括多重耐药菌株表现出裂解活性。
