Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, Australia.
Department of Surgery-Otolaryngology Head and Neck Surgery, The Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, South Australia, Australia.
Arch Microbiol. 2022 May 19;204(6):334. doi: 10.1007/s00203-022-02948-0.
Application of bacteriophages (phages) to treat complex multidrug-resistant bacterial infection is gaining traction because of its efficacy and universal availability. However, as phages are specific to their host, a diverse collection of locally isolated phage from various geographical locations is required to formulate a wide host range phage cocktail. Here, we report morphological and genomic features of three newly isolated phages from river water of the urban region in Kathmandu, Nepal, targeting three different bacteria (Escherichia coli, Klebsiella pneumoniae and Salmonella enterica.) from the Enterobacteriaceae family. Morphological identification and genome analysis indicated that two phages (Escherichia phage vB_EcoM_TU01 and Klebsiella phage vB_KpnP_TU02) were strictly lytic and free from integrases, virulence factors, toxins and known antimicrobial resistance genes, whereas Salmonella phage vB_SalS_TU03 was possibly a temperate phage. The genomic features of these phages indicate that natural phages are capable of lysing pathogenic bacteria and may have potential in bacterial biocontrol.
噬菌体（phages）在治疗复杂的多重耐药细菌感染方面的应用因其疗效和普遍适用性而受到关注。然而，由于噬菌体具有宿主特异性，因此需要从不同地理位置采集多种本地分离的噬菌体，以制备广谱噬菌体鸡尾酒。在这里，我们报告了从尼泊尔加德满都市区的河水分离到的三种新噬菌体的形态学和基因组特征，这些噬菌体针对肠杆菌科的三种不同细菌（大肠杆菌、肺炎克雷伯菌和肠炎沙门氏菌）。形态学鉴定和基因组分析表明，两种噬菌体（Escherichia phage vB_EcoM_TU01 和 Klebsiella phage vB_KpnP_TU02）是严格的裂解噬菌体，不含整合酶、毒力因子、毒素和已知的抗菌药物抗性基因，而沙门氏菌噬菌体 vB_SalS_TU03 可能是一种温和噬菌体。这些噬菌体的基因组特征表明，天然噬菌体能够裂解致病菌，可能具有细菌生物防治的潜力。
