Tisalema-Guanopatín Estefanía, Cabezas-Mera Fausto, Nolivos-Rodríguez Karla, Fierro Isabel, Pazmiño Lourdes, Garzon-Chavez Daniel, Debut Alexis, Vizuete Karla, Reyes Jorge Aníbal
Facultad de Ciencias Químicas, Universidad Central del Ecuador (UCE), Ciudadela Universitaria Avenida América, Quito, Pichincha, Ecuador.
Faculty of Engineering and Applied Sciences, Universidad Internacional SEK, Quito, Ecuador.
Phage (New Rochelle). 2023 Jun 1;4(2):99-107. doi: 10.1089/phage.2022.0039. Epub 2023 Jun 19.
Carbapenem-resistant , particularly isolates classified as sequence-type 258 (ST258), are multidrug-resistant strains that are strongly associated with poor-prognosis nosocomial infections, as current therapeutic options are limited and ineffective. In recent years, phage therapy has emerged as a promising treatment option for these scenarios.
We report the isolation and characterization of three new phages against ST258 strains recovered from Machángara river wastewater. These new members of the family showed stability over a wide temperature and pH range and burst sizes ranging from 6 to 44 plaque-forming units per bacteria. Their genomes were about 157 kilobases, with an average guanine-cytosine content of 46.4% and showed presence of several transfer RNAs, which also allowed us to predict a lytic replicative cycle due to the presence of endolysins and lysozymes.
Three lytic phages of family were recovered against ST258 strains from sewage; however, further characterization is needed for future consideration as therapeutic alternatives.
耐碳青霉烯类，特别是被归类为序列型258（ST258）的菌株，是多重耐药菌株，与预后不良的医院感染密切相关，因为目前的治疗选择有限且无效。近年来，噬菌体疗法已成为这些情况下一种有前景的治疗选择。
我们报告了从马钱加拉河废水中分离出的针对ST258菌株的三种新噬菌体的分离和特性。该噬菌体家族的这些新成员在很宽的温度和pH范围内表现出稳定性，每个细菌的裂解量在6到44个噬菌斑形成单位之间。它们的基因组约为157千碱基，鸟嘌呤-胞嘧啶平均含量为46.4%，并显示存在几种转运RNA，由于存在内溶素和溶菌酶，这也使我们能够预测其裂解复制周期。
从污水中分离出了针对ST258菌株的三种裂解性噬菌体；然而，作为治疗替代品，未来还需要进一步表征。
