Kunori T, Nishihira T, Tan M, Taguchi Y, Tsustumi E, Saito T, Saito H, Kasai M
Tohoku J Exp Med. 1978 Feb;124(2):161-8. doi: 10.1620/tjem.124.161.
Immunopathological investigations of the small round cells infiltrating into the tissues of human renal allografts and also those in urinary sediments were performed, using anti-human T-lymphocyte serum (AHTS). AHTS made from ALS is highly specific for T cells, but not for B cells. Tissues obtained by needle biopsy were examined by immunofluorescent staining and conventional histopathological methods. Urinary sediments were obtained from centrifugated fresh urine. Sediments were checked by immunofluorescent and Giemsa staining. Fluorescent positive cells were seen in the interstitial tissues and occasionally in renal glomeruli. Clustered cell-debris in the lumen of renal tubules was also stained by AHTS. In the urinary sediments, about a half of the lymphoic cells were stained by AHTS. The relationship between lymphocyturia and numerous infiltrating cells in the grafts during the rejection crisis has not been clarified. In this experiments, we tried to identify the detailed process of excretion of T cells to urine from renal tissues, using immunopathological methods.
利用抗人T淋巴细胞血清（AHTS）对浸润人肾移植组织及尿沉渣中的小圆形细胞进行了免疫病理学研究。由抗淋巴细胞血清制备的AHTS对T细胞具有高度特异性，而对B细胞则无特异性。通过针吸活检获取的组织采用免疫荧光染色和传统组织病理学方法进行检查。尿沉渣取自离心后的新鲜尿液。对沉渣进行免疫荧光和吉姆萨染色检查。在间质组织中可见荧光阳性细胞，偶尔在肾小球中也可见到。肾小管管腔内聚集的细胞碎片也被AHTS染色。在尿沉渣中，约一半的淋巴细胞被AHTS染色。移植排斥反应危机期间淋巴细胞尿与移植物中大量浸润细胞之间的关系尚未阐明。在本实验中，我们试图采用免疫病理学方法确定T细胞从肾组织排泄到尿液中的详细过程。
