Agarwal Preeti, Khurana Paramjit
Department of Plant Molecular Biology, University of Delhi South Campus, Dhaula Kuan, New Delhi, 110021, India.
Funct Integr Genomics. 2019 May;19(3):497-513. doi: 10.1007/s10142-019-00666-3. Epub 2019 Mar 13.
High temperature stress is known to be one of the major limiting factors for wheat productivity worldwide. HSFs are known to play a central role in heat stress response in plants. Hence, the current study is an attempt to explore an in-depth involvement of TaHSFs in stress responses mainly in heat and other abiotic responses like salinity, drought, and cold stress. Effort was made to understand as how the expression of HSF is able to define the differential robustness of wheat varieties. Subsequent studies were done to establish the involvement of any temporal or spatial cue on the behavior of these TaHSFs under heat stress conditions. A total of 53 HSFs have been reported until date and out of these, few TaHSFs including one identified in our library, i.e., TaHsfA2d (Traes_4AS_52EB860E7.2), were selected for the expression analysis studies. The expressions of these HSFs were found to differ in both magnitude and sensitivity to the heat as well as other abiotic stresses. Moreover, these TaHSFs displayed wide range of expression in different tissues like anther, ovary, lemma, palea, awn, glume, and different stages of seed development. Thus, TaHSFs appear to be under dynamic expression as they respond in a unique manner to spatial, temporal, and environmental cues. Therefore, these HSFs can be used as candidate genes for understanding the molecular mechanism under heat stress and can be utilized for improving crop yield by enhancing the tolerance and survival of the crop plants under adverse environment conditions.
高温胁迫是全球小麦产量的主要限制因素之一。已知热激转录因子（HSFs）在植物热应激反应中起核心作用。因此，本研究旨在深入探究TaHSFs在胁迫反应中的作用，主要包括热胁迫以及盐胁迫、干旱胁迫和冷胁迫等其他非生物胁迫反应。研究旨在了解HSF的表达如何决定小麦品种的不同抗逆性。随后进行了研究，以确定在热胁迫条件下，任何时间或空间线索对这些TaHSFs行为的影响。截至目前，共报道了53个HSFs，其中包括在我们的文库中鉴定出的几个TaHSFs，即TaHsfA2d（Traes_4AS_52EB860E7.2），被选用于表达分析研究。这些HSFs的表达在对热胁迫以及其他非生物胁迫的幅度和敏感性上均存在差异。此外，这些TaHSFs在花药、子房、内外稃、芒、颖片等不同组织以及种子发育的不同阶段均表现出广泛的表达。因此，TaHSFs在对空间、时间和环境线索作出独特反应时，其表达似乎处于动态变化中。所以，这些HSFs可作为候选基因，用于理解热胁迫下的分子机制，并通过提高作物在逆境条件下的耐受性和存活率来提高作物产量。
