Alghamdi A, Alattal Yehya
Abdullah Bagshan Chair for Bee Research, Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2021 Nov;28(11):6586-6591. doi: 10.1016/j.sjbs.2021.07.033. Epub 2021 Jul 16.
The Arabian Honeybee is endemic to the Arabian Peninsula. It is highly adapted to temperature extremes and drought dominating the region. In this study, the mitochondrial Cytochrome Oxidase I (COI) was analyzed in 133 specimens of A. m. jemenitica from eight localities along the Red Sea cost of Saudi Arabia. Results revealed 33 synonymous, and 6 non-synonymous mutations within the COI sequences, resulting in change of 4 amino acids. Phylogenetic analysis based on either type of mutations revealed two main haplogroups accounting for 94% of the samples. In total Eighteen new haplotypes were identified and uploaded in the genebank, Fourteen of them are restricted to one/both haplogroups. All haplotypes identified in this study clustered with reference COI sequences of the sub-lineag Z (African Lineage). However one Haplotype (MW428270) represents high COI variability compared to other haplotypes and may resemble different evolutionary sub-lineage. Tajima's Neutrality Test (Ps = 0.025; D = -1.5) indicated population size expansion that took place after selective sweep and/or purifying selection.
阿拉伯蜜蜂是阿拉伯半岛特有的物种。它高度适应该地区占主导地位的极端温度和干旱环境。在本研究中，对来自沙特阿拉伯红海沿岸八个地点的133个也门蜜蜂样本的线粒体细胞色素氧化酶I（COI）进行了分析。结果显示，COI序列中有33个同义突变和6个非同义突变，导致4个氨基酸发生变化。基于这两种类型突变的系统发育分析揭示了两个主要单倍群，占样本的94%。总共鉴定出18个新单倍型并上传至基因库，其中14个仅限于一个/两个单倍群。本研究中鉴定的所有单倍型均与亚谱系Z（非洲谱系）的参考COI序列聚类。然而，与其他单倍型相比，一个单倍型（MW428270）表现出较高的COI变异性，可能类似于不同的进化亚谱系。 Tajima中性检验（Ps = 0.025；D = -1.5）表明，在选择性清除和/或纯化选择之后，种群规模发生了扩张。
