miR-139-5p 通过靶向调控 MEX3A 抑制乳腺癌细胞的恶性行为
MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A.
机构信息
Department of General Surgery, The First People's Hospital of Yancheng, Yancheng, 224005 Jiangsu Province, China.
Yancheng First Hospital, Affiliated Hospital of Nanjing University Medical School, China.
出版信息
Comput Math Methods Med. 2021 Nov 2;2021:6591541. doi: 10.1155/2021/6591541. eCollection 2021.
OBJECTIVE
The study was designed to evaluate the underlying mechanism of microRNA-139-5p in breast cancer (BC).
METHODS
Expression statuses of microRNA-139-5p and MEX3A were measured by qRT-PCR and western blotting. The anticancer effect of microRNA-139-5p was tested by a set of assays. Interaction between microRNA-139-5p and MEX3A was validated by dual-luciferase detection.
RESULTS
MicroRNA-139-5p expression in BC cells was obviously low, while MEX3A was significantly overexpressed. MicroRNA-139-5p restrained proliferative, invasive, and migratory abilities of BC cells and increased apoptosis level of BC cells, while MEX3A exerted a promoting effect on BC cell growth. Dual-luciferase reporter detection confirmed that microRNA-139-5p bound to MEX3A 3'-UTR.
CONCLUSIONS
MicroRNA-139-5p inhibited the development of BC by targeting MEX3A. MicroRNA-139-5p/MEX3A may be a target for BC therapy.
目的
本研究旨在评估 microRNA-139-5p 在乳腺癌（BC）中的作用机制。
方法
通过 qRT-PCR 和 Western blot 检测 microRNA-139-5p 和 MEX3A 的表达状态。通过一系列实验测试 microRNA-139-5p 的抗癌作用。通过双荧光素酶检测验证 microRNA-139-5p 和 MEX3A 之间的相互作用。
结果
BC 细胞中的 microRNA-139-5p 表达明显降低，而 MEX3A 则显著过表达。microRNA-139-5p 抑制 BC 细胞的增殖、侵袭和迁移能力，并增加 BC 细胞的凋亡水平，而 MEX3A 则促进 BC 细胞的生长。双荧光素酶报告检测证实，microRNA-139-5p 结合到 MEX3A 的 3'-UTR 上。
结论
microRNA-139-5p 通过靶向 MEX3A 抑制 BC 的发展。microRNA-139-5p/MEX3A 可能是 BC 治疗的靶点。
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