Falkler W A, Martin S A, Vincent J W, Tall B D, Nauman R K, Suzuki J B
J Clin Periodontol. 1987 Jul;14(6):307-14. doi: 10.1111/j.1600-051x.1987.tb00976.x.
35 ANUG patients were examined and compared clinically and demographically. Plaque removed from ulcerated sites in 20 patients was cultured using quantitative anaerobic procedures and examined by electron and darkfield microscopy. Patients were classified as having ANUG when presenting with ulceration and necrosis of interproximal papillae, pain and bleeding. The clinical symptoms of fetid odor, pseudomembrane formation, lymphadenopathy and elevated body temperature were present in 97%, 85%, 61% and 39% of the ANUG patients, respectively. 83% of the patients were smokers. The ANUG patients demonstrated a lower average age (24 years) than the general clinic population (32 years). There was a slightly higher % of male (54%) than female (46%) and the % of Caucasian (51%) and black (49%) ANUG patients were almost equal. Cultural studies revealed that gram-negative rods were the predominant cultivable micro-organisms present in the plaque, representing 78.2% of the total recoverable count. Of these, nearly half were strict anaerobes with Bacteroides gingivalis and Fusobacterium nucleatum accounting for 7.8% and 3.4%, respectively. Anaerobic and facultative gram-positive cocci (15.5%), gram-negative cocci (3.5%) and gram-positive rods (2.8%) were also isolated. Microscopic analysis of the morphologic composition of plaque revealed that rods (43%) constituted the greatest % of the total microorganisms observed followed by spirochetes (30%) and cocci (27%). 8 distinct types of spirochetal periplasmic flagellar arrangement were observed by electron microscopy, the "2-4-2" periplasmic flagellar arrangement being most numerous.
对35例急性坏死性溃疡性龈炎（ANUG）患者进行了临床和人口统计学检查及比较。采用定量厌氧培养法对20例患者溃疡部位去除的菌斑进行培养，并通过电子显微镜和暗视野显微镜检查。当患者出现邻面乳头溃疡和坏死、疼痛及出血时，被分类为患有急性坏死性溃疡性龈炎。急性坏死性溃疡性龈炎患者中分别有97%、85%、61%和39%出现了口臭、假膜形成、淋巴结病和体温升高等临床症状。83%的患者为吸烟者。急性坏死性溃疡性龈炎患者的平均年龄（24岁）低于普通门诊人群（32岁）。男性患者比例（54%）略高于女性（46%），白种人（51%）和黑人（49%）急性坏死性溃疡性龈炎患者的比例几乎相等。培养研究表明，革兰氏阴性杆菌是菌斑中主要可培养微生物，占总可培养菌数的78.2%。其中，近一半为严格厌氧菌，牙龈拟杆菌和具核梭杆菌分别占7.8%和3.4%。还分离出了厌氧和兼性革兰氏阳性球菌（15.5%）、革兰氏阴性球菌（3.5%）和革兰氏阳性杆菌（2.8%）。对菌斑形态组成的显微镜分析显示，杆菌（43%）占观察到的总微生物的最大比例，其次是螺旋体（30%）和球菌（27%）。通过电子显微镜观察到8种不同类型的螺旋体周质鞭毛排列，其中“2-4-2”周质鞭毛排列最为常见。
