Zee K Y, Samaranayake L P, Attström R, Davies W I
Department of Periodontology, Faculty of Dentistry, University of Hong Kong, Hong Kong.
Arch Oral Biol. 1996 Jul;41(7):647-53. doi: 10.1016/s0003-9969(96)00065-9.
The aim of this study was to determine the predominant supragingival cultivable bacterial flora in Chinese individuals, using the experimental gingivitis model. A total of 11 healthy dental students, mean age 22.5 years (range 20-25) were recruited. All were provided with once-a-week dental prophylaxis and oral hygiene reinforcement for 3 weeks to ensure gingival health. In the fourth week, after prophylaxis, the participants entered a 14-day period without any plaque control. A plaque sample was collected at days 1, 3, 7 and 14 from the buccal surface of the upper right canine, second premolar, first premolar and first molar, respectively. Each sample was then dispensed in tryptic soy-broth transport medium and grown anaerobically to obtain pure cultures, which were subsequently identified. Results showed that Gram-positive cocci and rods were the predominant cultivatable species (51-61%) in the samples throughout the 14-day period; with time there was a decreasing percentage of cocci and an increasing percentage of rods. Gram-negative cocci and rods increased in proportion with the plaque age (11-37%). Streptococcus spp. were the predominant Gram-positive cocci while Actinomyces were the predominant Gram-positive rods isolated. Fusobacterium and Capnocytophaga spp. were the two most frequent Gram-negative anaerobic rods cultured. The results compared with those from other analogous studies from the West suggest the possibility of interracial differences in supragingival plaque flora.
本研究旨在利用实验性牙龈炎模型确定中国人群中主要的龈上可培养细菌菌群。共招募了11名健康牙科学生，平均年龄22.5岁（范围20 - 25岁）。所有参与者每周接受一次口腔预防治疗并加强口腔卫生护理，持续3周以确保牙龈健康。在第四周，预防治疗后，参与者进入为期14天的无菌斑控制期。分别在第1、3、7和14天从右上尖牙、第二前磨牙、第一前磨牙和第一磨牙的颊面采集菌斑样本。然后将每个样本接种于胰蛋白胨大豆肉汤运输培养基中，进行厌氧培养以获得纯培养物，随后对其进行鉴定。结果显示，在整个14天期间，革兰氏阳性球菌和杆菌是样本中主要的可培养菌种（51 - 61%）；随着时间推移，球菌的百分比下降，杆菌的百分比上升。革兰氏阴性球菌和杆菌的比例随菌斑年龄增加（11 - 37%）。链球菌属是主要的革兰氏阳性球菌，而放线菌是分离出的主要革兰氏阳性杆菌。梭杆菌属和二氧化碳嗜纤维菌属是培养出的两种最常见的革兰氏阴性厌氧杆菌。与来自西方的其他类似研究结果相比，这些结果表明龈上菌斑菌群可能存在种族差异。
