Dolan Laurie C, Ciliutti Paola, Bisini Laura, Marabottini Cristina, Curtin Brian
GRAS Associates, LLC 11810 Grand Park Avenue Suite 500, North Bethesda, MD, 20852, United States.
European Research Biology Center, S.r.l.Via Tito Speri 12/14, 00071, Pomezia RM, Italy.
Toxicol Rep. 2021 Oct 25;8:1814-1818. doi: 10.1016/j.toxrep.2021.10.012. eCollection 2021.
Results of genotoxicity studies for magnesium salts of isobutyrate and 2-methylbutyrate, two candidate ingredients for inclusion in animal feed, are described in this manuscript. Both substances were tested for mutagenicity in a bacterial reverse mutation assay and clastogenicity/aneugenicity in an micronucleus study in human lymphocytes, conducted according to Organisation for Economic Co-operation and Development (OECD) Guidelines. The substances were tested up to the limits of solubility in the tests. The results showed that that magnesium salts of isobutyrate and 2-methylbutyrate are not mutagenic, clastogenic or aneugenic. The tests were valid, as the negative and positive controls produced expected responses.
本文描述了异丁酸盐和2-甲基丁酸盐的镁盐这两种动物饲料候选成分的遗传毒性研究结果。根据经济合作与发展组织（OECD）指南，对这两种物质进行了细菌回复突变试验中的致突变性测试以及人淋巴细胞微核研究中的染色体断裂/非整倍体性测试。在测试中，对这两种物质进行了直至溶解度极限的测试。结果表明，异丁酸盐和2-甲基丁酸盐的镁盐没有致突变性、染色体断裂性或非整倍体性。由于阴性和阳性对照产生了预期的反应，所以测试是有效的。
