Burzlaff Arne, Beevers Carol, Pearce Helen, Lloyd Melvyn, Klipsch Kevin
EBRC Consulting GmbH, Raffaelstr. 4, 30177 Hannover, Germany.
Genetic Toxicology, Covance Laboratories Limited, Otley Road, Harrogate, North Yorkshire, HG3 1PY, UK.
Regul Toxicol Pharmacol. 2017 Jun;86:279-291. doi: 10.1016/j.yrtph.2017.03.018. Epub 2017 Mar 23.
The potential of molybdenum substances to cause genotoxic effects has been studied previously. However, a review of existing in vitro data, including an assessment of relevance and reliability, has shown that inconsistent results have been observed in the past. To resolve the inconsistencies, new studies were performed with the highly soluble sodium molybdate dihydrate according to OECD test guidelines. In a bacterial reverse mutation assay sodium molybdate dihydrate did not induce reverse mutations in five strains of Salmonella typhimurium. No mutagenic or clastogenic effect was observed at the tk locus of L5178Y mouse lymphoma cells. In a micronucleus test in cultured human peripheral blood lymphocytes no clastogenic or aneugenic effects were seen. These results can be read across to other inorganic molybdenum substances, that all release the molybdate ion [MoO] under physiological conditions as the only toxicologically relevant species. In summary, a weight of evidence assessment of all available in vitro data shows no evidence of genotoxicity of molybdenum substances.
钼物质导致遗传毒性效应的可能性此前已得到研究。然而，对现有体外数据的回顾，包括对相关性和可靠性的评估，表明过去观察到的结果并不一致。为解决这些不一致性问题，按照经合组织测试指南，使用高度可溶的二水合钼酸钠开展了新的研究。在细菌回复突变试验中，二水合钼酸钠未在五株鼠伤寒沙门氏菌中诱导回复突变。在L5178Y小鼠淋巴瘤细胞的tk位点未观察到诱变或致断裂效应。在培养的人外周血淋巴细胞微核试验中，未发现致断裂或非整倍体效应。这些结果可类推至其他无机钼物质，因为在生理条件下，所有这些物质均释放钼酸根离子[MoO]作为唯一具有毒理学相关性的物种。总之，对所有现有体外数据的证据权重评估表明，没有证据显示钼物质具有遗传毒性。
