Wang Wenliang, Wang Yansheng, Gong Zhiqing, Yang Shifa, Jia Fengjuan
Shandong Academy of Agricultural Science, Jinan, China.
Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.
Front Nutr. 2021 Oct 26;8:771757. doi: 10.3389/fnut.2021.771757. eCollection 2021.
(), regarded as an edible and medical mushroom, has attracted toward the research interests because of the high nutrition and bioactivity. The nutritional and medical properties of have been well-studied; however, research about the difference of the nutritional properties and transcriptome profiling between the two different harvesting periods of was limited. In this study, the nutritional properties and transcriptome profiling were compared between the two different harvesting periods of : AP_S1 (the stage for the first harvesting period) and AP_S2 (the stage for the third harvesting period). This study showed that AP_S1 had the more growth advantages than AP_S2 including biomass, auricle area and thickness, protein and calcium contents, and most species of the amino acid contents, which contributed to the higher sensory evaluation and acceptability of AP_S1. Transcriptome profiling showed that a total of 30,298 unigenes were successfully annotated in the two different harvesting periods of . At a threshold of two-fold change, 1,415 and 3,213 unigenes were up- and downregulated, respectively. All the differentially expressed genes (DEGs) analysis showed that the some synthesis and metabolic processes were strengthened in AP_S1, especially the synthesis and metabolism of the amino acids and protein. The enhanced energy metabolism pathways could provide more energy for AP_S1 to synthesize the nutritional substance. Moreover, the expressions of 10 selected DEGs involved in the amino acid and protein synthesis pathways and energy metabolism pathways were higher in AP_S1 compared to AP_S2, consistent with Illumina analysis. To the best of our knowledge, this is the first study that compares the nutritional properties and transcriptome profiling between the two different harvesting periods of and the results can present insights into the growth and genetic characteristics of .
（）作为一种可食用和药用的蘑菇，因其高营养和生物活性而吸引了研究兴趣。（）的营养和药用特性已得到充分研究；然而，关于（）两个不同采收期之间营养特性和转录组图谱差异的研究有限。在本研究中，比较了（）两个不同采收期：AP_S1（第一个采收期阶段）和AP_S2（第三个采收期阶段）的营养特性和转录组图谱。本研究表明，AP_S1比AP_S2具有更多的生长优势，包括生物量、耳片面积和厚度、蛋白质和钙含量以及大多数种类的氨基酸含量，这有助于提高AP_S1的感官评价和可接受性。转录组图谱显示，在（）的两个不同采收期共成功注释了30298个单基因。在两倍变化阈值下，分别有1415个和3213个单基因上调和下调。所有差异表达基因（DEG）分析表明，AP_S1中一些合成和代谢过程得到加强，尤其是氨基酸和蛋白质的合成与代谢。增强的能量代谢途径可以为AP_S1合成营养物质提供更多能量。此外，与AP_S2相比，AP_S1中参与氨基酸和蛋白质合成途径以及能量代谢途径的10个选定DEG的表达更高，与Illumina分析结果一致。据我们所知，这是第一项比较（）两个不同采收期营养特性和转录组图谱的研究，结果可以为（）的生长和遗传特征提供见解。
