Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China.
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Carbohydr Polym. 2015 May 20;122:39-45. doi: 10.1016/j.carbpol.2014.12.041. Epub 2015 Jan 3.
Mushroom extracts are a new source of supplements for health and pharmaceutical due to their bioactivities. This study was to optimize the extraction parameters of a soluble polysaccharide from Auricularia polytricha (SPAP) by response surface methodology. The practical optimum parameters were an extraction time of 4h, an extraction temperature of 95 °C and a ratio of water to fruiting bodies of 28 mL/g, and the highest extraction rate was 19.77%. In vivo, male Sprague-Dawley (SD) rats were diet-induced hypercholesterolemic models and oral administration of SPAP to evaluate anti-hypercholesterolemic effects. The results showed that SPAP decreased the serum concentrations of blood lipid, made them close to the normal level. The total cholesterol in the SPAP consumption groups was significantly decreased 34.6 ± 7.6% and 33.3 ± 7.9% with dose of 4.5 and 9.0mg/kg BW in the 29th day. This study suggested that SPAP was a suitable natural agent and may be applied in therapy.
由于其生物活性，蘑菇提取物是一种新的健康和制药补充剂来源。本研究旨在通过响应面法优化银耳（SPAP）可溶性多糖的提取参数。实际最佳参数为提取时间 4h、提取温度 95°C 和料液比 28mL/g，最高提取率为 19.77%。在体内，雄性 Sprague-Dawley（SD）大鼠被诱导为高胆固醇血症模型，并通过口服 SPAP 来评估其抗高胆固醇血症作用。结果表明，SPAP 降低了血脂的血清浓度，使其接近正常水平。在第 29 天，剂量为 4.5 和 9.0mg/kgBW 时，SPAP 消耗组的总胆固醇分别显著降低了 34.6±7.6%和 33.3±7.9%。本研究表明，SPAP 是一种合适的天然药物，可应用于治疗。
