Mugunthan Susithra Priyadarshni, Mani Chandra Harish
Plant Genetic Engineering and Molecular Farming Lab, Department of Biotechnology, Thiruvalluvar University, Vellore, India.
Front Vet Sci. 2021 Oct 26;8:721061. doi: 10.3389/fvets.2021.721061. eCollection 2021.
Avian mycoplasma is a bacterial disease causing chronic respiratory disease (CRD) in poultry industries with high economic losses. The eradication of this disease still remains as a challenge. A multi-epitope prophylactic vaccine aiming the antigenic proteins of can be a capable candidate to eradicate this infection. The present study is focused to design a multi-epitope vaccine candidate consisting of cytotoxic T-cell CTL), helper T-cell (HTL), and B-cell epitopes of antigenic proteins, using immunoinformatics strategies. The multi-epitopic vaccine was designed, and its tertiary model was predcited, which was further refined and validated by computational tools. After initial validation, molecular docking was performed between multi-epitope vaccine construct and chicken TLR-2 and 5 receptors, which predicted effective binding. The results specify the structural stability, precise specificity, and immunogenic response of the designed multi-epitope vaccine, and it could be an appropriate vaccine candidate for the infection.
禽支原体是一种在禽类产业中引发慢性呼吸道疾病（CRD）的细菌性疾病，会造成巨大经济损失。根除这种疾病仍然是一项挑战。一种针对[抗原蛋白名称未给出]抗原蛋白的多表位预防性疫苗可能是根除这种感染的有力候选疫苗。本研究的重点是利用免疫信息学策略设计一种由细胞毒性T细胞（CTL）、辅助性T细胞（HTL）和抗原蛋白的B细胞表位组成的多表位候选疫苗。设计了多表位疫苗，并预测了其三级模型，该模型通过计算工具进一步优化和验证。初步验证后，在多表位疫苗构建体与鸡TLR-2和5受体之间进行了分子对接，预测有有效结合。结果表明所设计的多表位疫苗具有结构稳定性、精确特异性和免疫原性反应，它可能是针对[疾病名称未给出]感染的合适候选疫苗。
