Mugunthan Susithra Priyadarshni, Harish Mani Chandra
Plant Genetic Engineering and Molecular Farming Lab, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, India.
ACS Omega. 2021 May 17;6(21):13742-13755. doi: 10.1021/acsomega.1c01032. eCollection 2021 Jun 1.
causes chronic respiratory disease in chickens leading to large economic losses in the poultry industry, and the impacts remain to be a great challenge for a longer period. Among the other approaches, a vaccine targeting the adhesion proteins of would be a promising candidate in controlling the infection. Thus, the present study is aimed to design a multi-epitope vaccine candidate using cytoadhesion proteins of through an advanced immunoinformatics approach. As a result, the multi-epitope vaccine was constructed, which comprised potential T-cell and B-cell binding epitopes with appropriate adjuvants. The designed multi-epitope vaccine represented high antigenicity with viable physiochemical properties. The prospective three-dimensional structure of the epitope was predicted, refined, and validated. The molecular docking analysis of multi-epitope vaccine candidates with the chicken Toll-like receptor-5 predicted effective binding. Furthermore, codon optimization and in silico cloning ensured high expression. Thus, the present finding indicates that the engineered multi-epitope vaccine is structurally stable and can induce a strong immune response. Furthermore, the multi-epitope vaccine is suggested to be a suitable vaccine candidate for the infection due to its effective binding capacity and precise specificity.
在鸡群中引发慢性呼吸道疾病，给家禽业造成巨大经济损失，且这种影响在较长时期内仍是巨大挑战。在其他方法中，针对[病原体名称]黏附蛋白的疫苗有望成为控制感染的候选方案。因此，本研究旨在通过先进的免疫信息学方法，利用[病原体名称]的细胞黏附蛋白设计一种多表位疫苗候选物。结果构建了多表位疫苗，其包含具有合适佐剂的潜在T细胞和B细胞结合表位。所设计的多表位疫苗具有高抗原性及可行的理化性质。预测、优化并验证了表位的三维结构。多表位疫苗候选物与鸡Toll样受体-5的分子对接分析预测了有效结合。此外，密码子优化和电子克隆确保了高表达。因此，本研究结果表明，工程化的多表位疫苗结构稳定，能诱导强烈免疫反应。此外，由于其有效的结合能力和精确的特异性，多表位疫苗被认为是针对[病原体名称]感染的合适疫苗候选物。
