Zubair Muhammad, Wang Jia, Yu Yanfei, Rasheed Muhammad Asif, Faisal Muhammad, Dawood Ali Sobhy, Ashraf Muhammad, Shao Guoqing, Feng Zhixin, Xiong Qiyan
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210000, China.
GuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou 225300, China.
Vet Sci. 2023 Sep 5;10(9):557. doi: 10.3390/vetsci10090557.
() is responsible for infections in the swine population. Such infections are usually cured by using antimicrobials and lead to develop resistance. Until now, there has been no effective vaccine to eradicate the disease. This study used conserved domains found in seven members of the variable lipoprotein (VlpA-G) family in order to design a multi-epitope candidate vaccine (MEV) against . The immunoinformatics approach was followed to predict epitopes, and a vaccine construct consisting of an adjuvant, two B cell epitopes, two HTL epitopes, and one CTL epitope was designed. The suitability of the vaccine construct was identified by its non-allergen, non-toxic, and antigenic nature. A molecular dynamic simulation was executed to assess the stability of the TLR2 docked structure. An immune simulation showed a high immune response toward the antigen. The protein sequence was reverse-translated, and codons were optimized to gain a high expression level in . The proposed vaccine construct may be a candidate for a multi-epitope vaccine. Experimental validation is required in future to test the safety and efficacy of the hypothetical candidate vaccine.
（某病原体）是猪群感染的病原体。此类感染通常通过使用抗菌药物治愈，并导致产生耐药性。到目前为止，尚无有效的疫苗来根除该疾病。本研究利用可变脂蛋白（VlpA - G）家族七个成员中发现的保守结构域，设计了一种针对（该病原体）的多表位候选疫苗（MEV）。采用免疫信息学方法预测表位，并设计了一种由佐剂、两个B细胞表位、两个辅助性T淋巴细胞（HTL）表位和一个细胞毒性T淋巴细胞（CTL）表位组成的疫苗构建体。通过其无过敏原、无毒和抗原性来确定疫苗构建体的适用性。进行了分子动力学模拟以评估Toll样受体2（TLR2）对接结构的稳定性。免疫模拟显示对抗原具有高免疫反应。对蛋白质序列进行反向翻译，并优化密码子以在（某种表达系统）中获得高表达水平。所提出的疫苗构建体可能是一种多表位疫苗的候选物。未来需要进行实验验证，以测试该假设候选疫苗的安全性和有效性。
