Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China.
Key Laboratory of Tropical Disease Control, Ministry of Education, Provincial Engineering Technology Research Center for Diseases-Vectors Control, Guangzhou, Guangdong, China.
Parasit Vectors. 2021 Nov 27;14(1):586. doi: 10.1186/s13071-021-05050-4.
Aedes aegypti and Aedes albopictus are invasive mosquito species and significantly impact human health in southern China. Microbiota are confirmed to affect the development and immunity of mosquitoes. However, scientists have focused more on midgut microbiota of female mosquitoes and bacterial differences between female and male Aedes mosquitoes. The relationship between the midgut and entire body microbiota of Aedes is unclear. In this study, we collected mosquito samples reared under the same laboratory conditions and compared the microbial composition of midgut and entire bodies of Aedes aegypti and Aedes albopictus using 16S rRNA gene sequencing.
In this study, we collected mosquito samples reared under the same laboratory conditions and compared the microbial composition of midgut and entire bodies of Aedes aegypti and Aedes albopictus using 16S rRNA gene sequencing.
A total of 341 OTUs were identified, showing that Proteobacteria was the dominant phylum and Methylobacterium the dominant genus in both Aedes aegypti and Aedes albopictus. The bacterial diversity and community structures of the entire bodies were similar between males and females in both Aedes aegypti and Aedes albopictus. Conversely, the bacterial compositions of male and female Aedes aegypti and Aedes albopictus were significantly different. NMDS analysis, UPGMA analysis, diversity indices and OTU distribution demonstrated that compositions and structures in midgut microbiota were similar but significantly different in the entire bodies of Aedes aegypti and Aedes albopictus. Functional prediction analysis showed that metabolism and environmental information processing were the dominant KEGG pathways at level 1. Our study showed that there were significantly different level 2 and 3 KEGG pathways in the midgut microbiota (16 level 2 and 24 level 3) and the entire bodies (33 level 2 and 248 level 3) between female Aedes albopictus and Aedes Aegypti.
Our findings that Aedes aegypti and Aedes albopictus reared in the same laboratory harbor a similar gut bacterial microbiome but different entire body microbiota imply that the gut microbiota of adult mosquitoes is environmentally determined regardless of the host genotype, but the entire body microbiota is more genetically determined. Our findings improved the understanding of the microbiota in the entire and partial tissues of Aedes mosquitoes.
埃及伊蚊和白纹伊蚊是入侵性蚊种，对中国南方的人类健康有重大影响。微生物群已被证实会影响蚊子的发育和免疫力。然而，科学家们更关注雌性蚊子的中肠微生物群以及雌性和雄性埃及伊蚊和白纹伊蚊之间的细菌差异。关于埃及伊蚊和白纹伊蚊中肠和整个身体微生物群之间的关系尚不清楚。在这项研究中，我们收集了在相同实验室条件下饲养的蚊子样本，并使用 16S rRNA 基因测序比较了埃及伊蚊和白纹伊蚊中肠和整个身体的微生物组成。
在这项研究中，我们收集了在相同实验室条件下饲养的蚊子样本，并使用 16S rRNA 基因测序比较了埃及伊蚊和白纹伊蚊中肠和整个身体的微生物组成。
共鉴定出 341 个 OTUs，结果表明，在埃及伊蚊和白纹伊蚊中，变形菌门是优势门，甲基杆菌属是优势属。埃及伊蚊和白纹伊蚊雌雄个体的整个身体的细菌多样性和群落结构相似。相反，埃及伊蚊和白纹伊蚊雌雄个体的细菌组成有显著差异。NMDS 分析、UPGMA 分析、多样性指数和 OTU 分布表明，中肠微生物群的组成和结构相似，但埃及伊蚊和白纹伊蚊整个身体的结构明显不同。功能预测分析显示，代谢和环境信息处理是水平 1 中主要的 KEGG 途径。我们的研究表明，雌性白纹伊蚊和埃及伊蚊中肠微生物群（16 个水平 2 和 24 个水平 3）和整个身体（33 个水平 2 和 248 个水平 3）之间存在显著不同的水平 2 和 3 KEGG 途径。
我们的研究结果表明，在相同实验室中饲养的埃及伊蚊和白纹伊蚊携带相似的肠道细菌微生物组，但整个身体的微生物组不同，这表明成年蚊子的肠道微生物群是由环境决定的，而与宿主基因型无关，但整个身体的微生物群更多地由遗传决定。我们的研究结果提高了对埃及伊蚊和白纹伊蚊整个和部分组织中微生物组的认识。
