Cvek M, Granath L, Cleaton-Jones P, Austin J
J Dent Res. 1987 Jun;66(6):1166-74. doi: 10.1177/00220345870660061501.
Monkey incisor teeth were pulpotomized in groups of 10. After physiological hemostasis, the pulps of group I were covered with isobutyl cyanoacrylate, and those of groups II and III with calcium hydroxide for 10 and 60 minutes, respectively, whereafter this compound was washed away and the wound surfaces covered with Teflon. In group IV, calcium hydroxide was used as a positive control, and Teflon as a negative control in group V. The animals were killed after 12 weeks and the teeth removed in tissue blocks. The material was processed and evaluated histologically with respect to location and continuity of a hard tissue barrier, type of newly formed hard tissue, state of the pulp, and presence of stainable bacteria in the coronal cavity. Seven of nine teeth in group I showed a hard tissue barrier. The corresponding figure for group II was eight out of 10 teeth. All teeth in groups III and IV had a barrier. The incidence of a continuous barrier increased from group I through group IV, as did the incidence of its location below the level of the original wound surface. The condition of the pulp was related to the presence of bacteria and the continuity of the barrier to the presence of inflammation. There was no bridging in group V. The results support the theory that a low-grade irritation is responsible for the formation of a hard tissue barrier in exposed pulps.
将10只猴子分为一组，对其门齿进行牙髓切断术。生理止血后，第一组牙髓覆盖氰基丙烯酸异丁酯，第二组和第三组牙髓分别用氢氧化钙覆盖10分钟和60分钟，之后洗去该化合物，创面覆盖聚四氟乙烯。第四组以氢氧化钙作为阳性对照，第五组以聚四氟乙烯作为阴性对照。12周后处死动物，取出牙齿制成组织块。对材料进行处理，并从硬组织屏障的位置和连续性、新形成硬组织的类型、牙髓状态以及冠部腔隙中可染色细菌的存在情况等方面进行组织学评估。第一组9颗牙齿中有7颗显示有硬组织屏障。第二组相应的数据是10颗牙齿中有8颗。第三组和第四组所有牙齿都有屏障。从第一组到第四组，连续屏障的发生率增加，其位于原始创面水平以下的发生率也增加。牙髓状况与细菌的存在有关，而屏障的连续性与炎症的存在有关。第五组没有桥接现象。结果支持这样一种理论，即低度刺激是暴露牙髓中硬组织屏障形成的原因。
