State Key Laboratory for Managing Biotic and Chemical Threats To the Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.
School of Resources and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou, 341000, China.
Appl Microbiol Biotechnol. 2021 Dec;105(24):9371-9383. doi: 10.1007/s00253-021-11666-4. Epub 2021 Nov 12.
The microbial community within the root system, the rhizosphere closely connected to the root, and their symbiotic relationship with the host are increasingly seen as possible drivers of natural pathogen resistance. Resistant cultivars have the most effective strategy in controlling the Chinese wheat yellow mosaic disease, but the roles of the root and rhizosphere microbial interactions among different taxonomic levels of resistant cultivars are still unknown. Thus, we aimed to investigate whether these microbial community composition and network characteristics are related to disease resistance and to analyze the belowground plant-associated microflora. Relatively high microbial diversity and stable community structure for the resistant cultivars were detected. Comparison analysis showed that some bacterial phyla were significantly enriched in the wheat root or rhizosphere of the resistant wheat cultivar. Furthermore, the root and rhizosphere of the resistant cultivars greatly recruited many known beneficial bacterial and fungal taxa. In contrast, the relative abundance of potential pathogens was higher for the susceptible cultivar than for the resistant cultivar. Network co-occurrence analysis revealed that a much more complex, more mutually beneficial, and a higher number of bacterial keystone taxa in belowground microbial networks were displayed in the resistant cultivar, which may have been responsible for maintaining the stability and ecological balance of the microbial community. Overall, compared with the susceptible cultivar, the resistant cultivar tends to recruit more potential beneficial microbial groups for plant and rhizosphere microbial community interactions. These findings indicate that beneficial rhizosphere microbiomes for cultivars should be targeted and evaluated using community compositional profiles. KEY POINTS: • Different resistance levels in cultivars affect the rhizosphere microbiome.. • Resistant cultivars tend to recruit more potential beneficial microbial groups. • Bacteria occupy a high proportion and core position in the microflora network.
根系内部的微生物群落、与根系紧密相连的根际，以及它们与宿主的共生关系，正逐渐被视为天然病原体抗性的可能驱动因素。具有抗性的品种在控制中国小麦黄花叶病方面具有最有效的策略，但不同分类水平的抗性品种的根际微生物相互作用的作用尚不清楚。因此，我们旨在研究这些微生物群落组成和网络特征是否与抗性有关，并分析地下植物相关微生物群。抗性品种检测到相对较高的微生物多样性和稳定的群落结构。比较分析表明，一些细菌门在抗性小麦品种的根或根际中明显富集。此外，抗性品种的根和根际极大地招募了许多已知的有益细菌和真菌类群。相比之下，感病品种的潜在病原体相对丰度高于抗性品种。网络共现分析表明，抗性品种的地下微生物网络中显示出更多复杂、相互有益和更多数量的细菌关键类群，这可能有助于维持微生物群落的稳定性和生态平衡。总体而言，与感病品种相比，抗性品种倾向于招募更多潜在有益的微生物群体，用于植物和根际微生物群落相互作用。这些发现表明，应该针对具有抗性的品种，并根据群落组成特征来评估有益的根际微生物组。关键点：
品种的不同抗性水平影响根际微生物群落。
抗性品种倾向于招募更多潜在有益的微生物群体。
细菌在微生物网络中占据高比例和核心地位。
