Adolpho Leticia Faustino, Ribeiro Larissa Mayra Silva, Freitas Gileade Pereira, Lopes Helena Bacha, Gomes Maria Paula Oliveira, Ferraz Emanuela Prado, Gimenes Rossano, Beloti Marcio Mateus, Rosa Adalberto Luiz
Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, Brazil.
Institute of Physics and Chemistry, University of Itajubá, Itajubá 37500-903, MG, Brazil.
J Funct Biomater. 2023 Jun 1;14(6):306. doi: 10.3390/jfb14060306.
Tissue engineering and cell therapy have been the focus of investigations on how to treat challenging bone defects. This study aimed to produce and characterize a P(VDF-TrFE)/BaTiO scaffold and evaluate the effect of mesenchymal stem cells (MSCs) combined with this scaffold and photobiomodulation (PBM) on bone repair.
P(VDF-TrFE)/BaTiO was synthesized using an electrospinning technique and presented physical and chemical properties suitable for bone tissue engineering. This scaffold was implanted in rat calvarial defects (unilateral, 5 mm in diameter) and, 2 weeks post-implantation, MSCs were locally injected into these defects ( = 12/group). Photobiomodulation was then applied immediately, and again 48 and 96 h post-injection. The μCT and histological analyses showed an increment in bone formation, which exhibited a positive correlation with the treatments combined with the scaffold, with MSCs and PBM inducing more bone repair, followed by the scaffold combined with PBM, the scaffold combined with MSCs, and finally the scaffold alone (ANOVA, ≤ 0.05).
The P(VDF-TrFE)/BaTiO scaffold acted synergistically with MSCs and PBM to induce bone repair in rat calvarial defects. These findings emphasize the need to combine a range of techniques to regenerate large bone defects and provide avenues for further investigations on innovative tissue engineering approaches.
组织工程和细胞治疗一直是关于如何治疗具有挑战性的骨缺损研究的重点。本研究旨在制备并表征一种聚（偏二氟乙烯-三氟乙烯）/钛酸钡支架，并评估间充质干细胞（MSCs）与该支架及光生物调节（PBM）联合应用对骨修复的影响。
采用静电纺丝技术合成聚（偏二氟乙烯-三氟乙烯）/钛酸钡，其呈现出适合骨组织工程的物理和化学性质。将该支架植入大鼠颅骨缺损处（单侧，直径5毫米），植入后2周，将间充质干细胞局部注入这些缺损处（每组n = 12）。然后立即进行光生物调节，并在注射后48小时和96小时再次进行。μCT和组织学分析显示骨形成增加，这与支架联合治疗呈正相关，间充质干细胞与光生物调节联合诱导更多的骨修复，其次是支架与光生物调节联合、支架与间充质干细胞联合，最后是单独的支架（方差分析，P≤0.05）。
聚（偏二氟乙烯-三氟乙烯）/钛酸钡支架与间充质干细胞和光生物调节协同作用，诱导大鼠颅骨缺损处的骨修复。这些发现强调了结合一系列技术来再生大的骨缺损的必要性，并为进一步研究创新的组织工程方法提供了途径。
