Department of Reproductive Medicine, Xiangyang NO.1 People's Hospital, Hubei University of Medicine, Xiangyang, China.
Department of Urology, Chengdu Fifth People's Hospital, Chengdu, China.
DNA Cell Biol. 2021 Nov;40(11):1445-1455. doi: 10.1089/dna.2021.0416.
Metastatic prostate cancer (PCa) has become a major obstacle in the treatment of PCa. The study's purpose is to find biomarkers of tumor metastasis by proteomics and enzyme-linked immunosorbent assay (ELISA), and to design related experiments to study its role in the progress and metastasis of PCa. We analyzed serum from primary PCa stage and metastatic stage of 12 patients to find metastatic PCa serum protein biomarkers using isobaric tags for relative and absolute quantitation (iTRAQ). An effective diagnostic model based on validated biomarkers using logistic regression was established. and biological behavior experiments (wound healing, CCK8, and Transwell tests) were carried out after obtaining the biomarkers. Related mechanism has been studied, which may be associated with metastatic PCa. Actin gamma 1 () is a potential biomarker in the metastasis of PCa. Bioinformatics and related experiments show that is high-expressed in PCa tissues and cells. and experiments illustrated that the ability of proliferation, migration, and invasion of PCa cells was significantly inhibited after the knockdown of expression. Surprisingly, ERK protein expression was downregulated after knockdown. At the same time, the expression of epithelial-mesenchymal transition-related markers in PCa cells decrease after treated with ERK1/2 inhibitor, which indicating that may affect the metastatic ability of PCa cells through signaling pathway. is a marker of metastasis PCa. It mediates cell proliferation and may regulate the metastasis of PCa through signaling pathway, which provides a useful theoretical basis for exploring the treatment of PCa.
转移性前列腺癌（PCa）已成为 PCa 治疗的主要障碍。本研究旨在通过蛋白质组学和酶联免疫吸附试验（ELISA）寻找肿瘤转移的生物标志物，并设计相关实验研究其在 PCa 进展和转移中的作用。我们分析了 12 例患者的原发 PCa 期和转移期的血清，使用同位素相对和绝对定量（iTRAQ）技术寻找转移性 PCa 血清蛋白生物标志物。使用逻辑回归建立了基于验证生物标志物的有效诊断模型。并获得生物标志物后进行了生物学行为实验（划痕愈合、CCK8 和 Transwell 试验）。研究了相关机制，可能与转移性 PCa 有关。肌动蛋白γ 1（）是 PCa 转移的潜在生物标志物。生物信息学和相关实验表明，在 PCa 组织和细胞中高表达。并且实验表明，敲低表达后，PCa 细胞的增殖、迁移和侵袭能力显著受到抑制。令人惊讶的是，ERK 蛋白表达在 敲低后下调。同时，ERK1/2 抑制剂处理后 PCa 细胞中上皮-间充质转化相关标志物的表达降低，这表明可能通过 信号通路影响 PCa 细胞的转移能力。是转移性 PCa 的标志物。它介导细胞增殖，可能通过 信号通路调节 PCa 的转移，为探索 PCa 的治疗提供了有用的理论依据。
