Department of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Science, Postępu 36A, 05-552 Magdalenka, Poland.
Department of Microwave Safety, Military Institute of Hygiene and Epidemiology, 01-163 Warsaw, Poland.
Int J Mol Sci. 2021 Oct 27;22(21):11605. doi: 10.3390/ijms222111605.
The invention of non-ionizing emission devices revolutionized science, medicine, industry, and the military. Currently, different laser systems are commonly used, generating the potential threat of excessive radiation exposure, which can lead to adverse health effects. Skin is the organ most exposed to laser irradiation; therefore, this study aims to evaluate the effects of 445 nm, 520 nm, and 638 nm non-ionizing irradiation on keratinocytes and fibroblasts. Keratinocytes and fibroblasts were exposed to a different fluency of 445 nm, 520 nm, and 638 nm laser irradiation. In addition, viability, type of cell death, cell cycle distribution, and proliferation rates were investigated. The 445 nm irradiation was cytotoxic to BJ-5ta (≥58.7 J/cm) but not to Ker-CT cells. Exposure influenced the cell cycle distribution of Ker-CT (≥61.2 J/cm) and BJ-5ta (≥27.6 J/cm) cells, as well as the Bj-5ta proliferation rate (≥50.5 J/cm). The 520 nm irradiation was cytotoxic to BJ-5ta (≥468.4 J/cm) and Ker-CT (≥385.7 J/cm) cells. Cell cycle distribution (≥27.6 J/cm) of Ker-CT cells was also affected. The 638 nm irradiation was cytotoxic to BJ-5ta and Ker-CT cells (≥151.5 J/cm). The proliferation rate and cell cycle distribution of BJ-5ta (≥192.9 J/cm) and Ker-CT (13.8 and 41.3 J/cm) cells were also affected. At high fluences, 455 nm, 520 nm, and 638 nm irradiation, representing blue, green, and red light spectra, are hazardous to keratinocytes and fibroblasts. However, laser irradiation may benefit the cells at low fluences by modulating the cell cycle and proliferation rate.
非电离发射装置的发明彻底改变了科学、医学、工业和军事领域。目前，不同的激光系统通常被使用，产生潜在的过度辐射暴露的威胁，这可能导致不良的健康影响。皮肤是最容易受到激光照射的器官；因此，本研究旨在评估 445nm、520nm 和 638nm 非电离照射对角质形成细胞和成纤维细胞的影响。将角质形成细胞和成纤维细胞暴露于不同的 445nm、520nm 和 638nm 激光照射下。此外，还研究了细胞活力、细胞死亡类型、细胞周期分布和增殖率。445nm 照射对 BJ-5ta（≥58.7J/cm）具有细胞毒性，但对 Ker-CT 细胞没有影响。照射影响了 Ker-CT（≥61.2J/cm）和 BJ-5ta（≥27.6J/cm）细胞的细胞周期分布，以及 Bj-5ta 的增殖率（≥50.5J/cm）。520nm 照射对 BJ-5ta（≥468.4J/cm）和 Ker-CT（≥385.7J/cm）细胞具有细胞毒性。Ker-CT 细胞的细胞周期分布（≥27.6J/cm）也受到影响。638nm 照射对 BJ-5ta 和 Ker-CT 细胞具有细胞毒性（≥151.5J/cm）。BJ-5ta（≥192.9J/cm）和 Ker-CT（13.8 和 41.3J/cm）细胞的增殖率和细胞周期分布也受到影响。在高辐照下，455nm、520nm 和 638nm 照射，代表蓝、绿和红光光谱，对角质形成细胞和成纤维细胞有危害。然而，激光照射在低辐照下可能通过调节细胞周期和增殖率对细胞有益。
