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在 中鉴定和精细定位一个与叶片上卷性状相关的基因（Bnuc3）。

Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in .

机构信息

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.

Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Int J Mol Sci. 2021 Oct 28;22(21):11693. doi: 10.3390/ijms222111693.

DOI:10.3390/ijms222111693
PMID:34769127
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8583815/
Abstract

Leaf trait is an important target trait in crop breeding programs. Moderate leaf curling may be a help for improving crop yield by minimizing the shadowing by leaves. Mining locus for leaf curling trait is of significance for plant genetics and breeding researches. The present study identified a novel rapeseed accession with up-curling leaf, analyzed the up-curling leaf trait inheritance, and fine mapped the locus for up-curling leaf property ( in . Genetic analysis revealed that the up-curling leaf trait is controlled by a single dominant locus, named . We performed an association study of with single nucleotide polymorphism (SNP) markers using a backcross population derived from the homozygous up-curling leaf line NJAU-M1295 and the canola variety 'zhongshuang11' with typical flat leaves, and mapped the locus in a 1.92 Mb interval of chromosome A02 of . To further map , 232 simple sequence repeat (SSR) primers and four pairs of Insertion/Deletion (InDel) primers were developed for the mapping interval. Among them, five SSR markers and two InDel markers were polymorphic. By these markers, the mapping interval was narrowed to 92.0 kb using another F population. This fine mapping interval has 11 annotated genes among which were inferred to be candidate casual genes for up-curling leaf based on the cloned sequence analysis, gene functionality, and gene expression analysis. The current study laid a foundational basis for further elucidating the mechanism of and breeding of variety with up-curling leaf.

摘要

叶片特性是作物育种计划中的一个重要目标性状。适度的叶片卷曲可以通过最小化叶片的阴影来帮助提高作物产量。挖掘叶片卷曲性状的基因座对于植物遗传学和育种研究具有重要意义。本研究鉴定了一个新型的油菜卷曲叶品系，分析了卷曲叶性状的遗传，精细定位了卷曲叶特性的基因座（命名为 ）。遗传分析表明，卷曲叶性状由一个单显性基因座控制。我们使用来自纯合卷曲叶系 NJAU-M1295 和典型平展叶油菜品种‘中双 11’的回交群体，对 与单核苷酸多态性（SNP）标记进行了关联研究，将 基因座定位在 染色体 A02 的 1.92 Mb 区间内。为了进一步定位 ，在图谱区间内设计了 232 个简单重复序列（SSR）引物和 4 对插入/缺失（InDel）引物。其中，有 5 个 SSR 标记和 2 个 InDel 标记具有多态性。利用另一个 F2 群体，通过这些标记将图谱区间缩小到 92.0 kb。这个精细定位区间有 11 个注释基因，其中根据克隆序列分析、基因功能和基因表达分析，推测 是卷曲叶的候选基因。本研究为进一步阐明 基因的作用机制和培育卷曲叶品种奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4d/8583815/78f48a28678f/ijms-22-11693-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4d/8583815/aa800771bf90/ijms-22-11693-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4d/8583815/b5d6c55cb9cd/ijms-22-11693-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4d/8583815/cd25f1ffb4f4/ijms-22-11693-g003.jpg
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