免疫组织化学分析 Beclin-1 的表达预测口腔鳞状细胞癌的进展。
Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma.
机构信息
Unit of Maxillofacial Surgery, Department of Experimental and Clinical Medicine, "Magna Graecia" University, Viale Europa, 88100 Catanzaro, Italy.
Pathology Unit, Department of Health Science, Medical School, Magna Græcia University of Catanzaro, Viale Europa, 88100 Catanzaro, Italy.
出版信息
Int J Environ Res Public Health. 2021 Oct 22;18(21):11125. doi: 10.3390/ijerph182111125.
BACKGROUND
Autophagy is a cellular process responsible for maintaining homeostasis; a dysregulation of this process is involved in the development and progression of neoplasms. Beclin-1 is one of the major proteins linked to autophagy. However, the data regarding the association between the role of Beclin-1 and the progression of Oral Squamous Cell Carcinoma (OSCC) are rather low. For this reason, the objective of this study is to evaluate, through immunohistochemical techniques, the prognostic role of the expression of Beclin-1 autophagy marker in patients with OSCC.
METHODS
This is a single-centre retrospective study that includes patients with OSCC admitted to the Maxillofacial Unit of "Magna Graecia" University between January 2019 and September 2020. All the samples obtained from surgery were treated with anti Beclin-1 antibodies and subjected to immunohistochemical methods.
RESULTS
A total of 26 samples were analysed and the following variables were evaluated for each: percentage of positive Beclin-1 expression by tumour cells, signal strength of tumour cells, and total score. The variables considered were first normalised according to the D'Agostino and Pearson test, then analysed using the Pearson linear correlation coefficient: a statistically significant correlation was found between the parameters infiltration-intensity ( = 0.0088), infiltration-percent ( = 0.0123), intensity-total score ( = 0.0060).
CONCLUSIONS
The immunohistochemical evaluation of Beclin-1 revealed a statistically significant correlation between the intensity of the molecule's expression and a greater degree of infiltration of the neoplasm. Beclin-1 can, therefore, be considered a valid prognostic index of disease.
背景
自噬是一种负责维持细胞内环境稳定的细胞过程；该过程的失调与肿瘤的发生和发展有关。Beclin-1 是与自噬相关的主要蛋白之一。然而，关于 Beclin-1 作用与口腔鳞状细胞癌（OSCC）进展之间的关联的数据相当有限。出于这个原因，本研究的目的是通过免疫组织化学技术评估 Beclin-1 自噬标志物在 OSCC 患者中的预后作用。
方法
这是一项单中心回顾性研究，纳入了 2019 年 1 月至 2020 年 9 月在“Magna Graecia”大学颌面外科接受治疗的 OSCC 患者。从手术中获得的所有样本均用抗 Beclin-1 抗体处理，并进行免疫组织化学方法检测。
结果
共分析了 26 个样本，并对每个样本的肿瘤细胞中 Beclin-1 表达的阳性百分比、肿瘤细胞的信号强度和总评分进行了评估。根据 D'Agostino 和 Pearson 检验对变量进行了归一化，然后使用 Pearson 线性相关系数进行分析：浸润强度（ = 0.0088）、浸润百分比（ = 0.0123）和强度总评分（ = 0.0060）之间存在统计学显著相关性。
结论
Beclin-1 的免疫组织化学评估显示，该分子表达强度与肿瘤浸润程度之间存在统计学显著相关性。因此，Beclin-1 可以被认为是疾病的有效预后指标。
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