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HPLC-DAD-qTOF 分析食品加工中提取的粗番茄蛋白提取物中酚类化合物的组成。

HPLC-DAD-qTOF Compositional Analysis of the Phenolic Compounds Present in Crude Tomato Protein Extracts Derived from Food Processing.

机构信息

Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, Glagoljaška 8, SI-6000 Koper, Slovenia.

The Biocomposites Centre, Bangor University, Bangor LL57 2DG, UK.

出版信息

Molecules. 2021 Oct 23;26(21):6403. doi: 10.3390/molecules26216403.

Abstract

The conversion of raw fruits and vegetables, including tomatoes into processed food products creates side streams of residues that can place a burden on the environment. However, these processed residues are still rich in bioactive compounds and in an effort to valorize these materials in tomato by-product streams, the main aim of this study is to extract proteins and identify the main phenolic compounds present in tomato pomace (TP), peel and skins (TPS) by HPLC-DAD-ESI-QTOF. Forty different phenolic compounds were identified in the different tomato extracts, encompassing different groups of phenolic compounds, including derivatives of simple phenolic acid derivatives, hydroxycinnamoylquinic acid, flavones, flavonones, flavonol, and dihydrochalcone. In the crude protein extract (TPE) derived from tomatoes, most of these compounds were still present, confirming that valuable phenolic compounds were not degraded during food processing of these co-product streams. Moreover, phenolic compounds present in the tomato protein crude extract could provide a valuable contribution to the required daily intake of phenolics that are usually supplied by consuming fresh vegetables and fruits.

摘要

将生的水果和蔬菜(包括西红柿)加工成食品会产生副产物残渣,这可能会给环境带来负担。然而,这些加工后的残渣仍然富含生物活性化合物。为了充分利用番茄加工副产物中的这些材料,本研究的主要目的是从番茄渣(TP)、果皮和表皮(TPS)中提取蛋白质,并通过 HPLC-DAD-ESI-QTOF 鉴定其中存在的主要酚类化合物。在不同的番茄提取物中鉴定出了 40 种不同的酚类化合物,涵盖了不同类别的酚类化合物,包括简单酚酸衍生物、羟基肉桂酰奎宁酸、类黄酮、黄酮醇、黄酮醇和二氢查耳酮的衍生物。在从番茄中提取的粗蛋白提取物(TPE)中,这些化合物中的大部分仍然存在,这证实了在这些副产物流的食品加工过程中,有价值的酚类化合物并没有被降解。此外,番茄粗蛋白提取物中存在的酚类化合物可能对通常通过食用新鲜蔬菜和水果来获得的每日所需酚类化合物摄入量有重要贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/438b/8587773/54667d65dec5/molecules-26-06403-g001.jpg

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