Postgraduate Program of Pharmaceutical Innovation, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, Brazil.
Postgraduate Program in Health Sciences, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, Brazil.
Molecules. 2021 Oct 25;26(21):6439. doi: 10.3390/molecules26216439.
This work aimed to prepare a nanoemulsion containing the essential oil of the resin and evaluate its biocidal activities against the different stages of development of the mosquito. Ovicide, pupicide, adulticide and repellency assays were performed. The main constituents were -cymene (27.70%) and -pinene (22.31%). The developed nanoemulsion showed kinetic stability and monomodal distribution at a hydrophilic-lipophilic balance of 14 with a droplet size of 115.56 ± 1.68 nn and a zeta potential of -29.63 ± 3.46 mV. The nanoemulsion showed insecticidal action with LC 0.404 µg·mL for the ovicidal effect. In the pupicidal test, at the concentration of 160 µg·mL, 100% mortality was reached after 24 h. For adulticidal activity, a diagnostic concentration of 200 µg·mL (120 min) was determined. In the repellency test, a concentration of 200 µg·mL during the 180 min of the test showed a protection index of 77.67%. In conclusion, the nanobiotechnological product derived from the essential oil of resin can be considered as a promising colloid that can be used to control infectious disease vectors through a wide range of possible modes of applications, probably as this bioactive delivery system may allow the optimal effect of the terpenes in aqueous media and may also induce satisfactory delivery to air interfaces.
本工作旨在制备含有树脂精油的纳米乳剂，并评估其对 蚊不同发育阶段的杀菌活性。进行了杀卵、杀幼虫、杀成虫和驱避作用试验。主要成分为 - 柠檬烯（27.70%）和 - 蒎烯（22.31%）。所开发的纳米乳剂在亲水 - 亲油平衡值为 14 时表现出动力学稳定性和单模态分布，粒径为 115.56 ± 1.68 nn，zeta 电位为-29.63 ± 3.46 mV。纳米乳剂表现出杀虫作用，LC 0.404 µg·mL 对杀卵效果。在杀幼虫试验中，在 160 µg·mL 的浓度下，24 小时后达到 100%死亡率。对于成虫活性，确定了诊断浓度为 200 µg·mL（120 分钟）。在驱避试验中，在 180 分钟的试验中，浓度为 200 µg·mL 时的保护指数为 77.67%。总之，来源于树脂精油的纳米生物技术产品可以被认为是一种有前途的胶体，可通过多种可能的应用模式来控制传染病媒介，可能是因为这种生物活性传递系统可以允许萜烯在水介质中发挥最佳效果，也可以诱导对空气界面的令人满意的传递。
