Institute of Natural Fibres and Medicinal Plants, National Research Institute, Wojska Polskiego 71B, 60-630 Poznan, Poland.
Molecules. 2021 Oct 26;26(21):6467. doi: 10.3390/molecules26216467.
Nowadays, more and more attention is paid to the development and the intensification of the use of renewable energy sources. Hemp might be an alternative plant for bioenergy production. In this paper, four varieties of Polish industrial hemp (Białobrzeskie, Tygra, Henola, and Rajan) were investigated in order to determine which of them are the most advantageous raw materials for the effective production of bioethanol. At the beginning, physical and chemical pretreatment of hemp biomass was carried out. It was found that the most effective is the alkaline treatment with 2% NaOH, and the biomasses of the two varieties were selected for next stages of research: Tygra and Rajan. Hemp biomass before and after pretreatment was analyzed by FTIR and SEM, which confirmed the effectiveness of the pretreatment. Next, an enzymatic hydrolysis process was carried out on the previously selected parameters using the response surface methodology. Subsequently, the two approaches were analyzed: separated hydrolysis and fermentation (SHF) and a simultaneous saccharification and fermentation (SSF) process. For Tygra biomass in the SHF process, the ethanol concentration was 10.5 g∙L (3.04 m·ha), and for Rajan biomass at the SSF process, the ethanol concentration was 7.5 g∙L (2.23 m·ha). In conclusion, the biomass of Polish varieties of hemp, i.e., Tygra and Rajan, was found to be an interesting and promising raw material for bioethanol production.
如今，人们越来越关注可再生能源的开发和利用。大麻可能是生物能源生产的替代植物。本文研究了波兰四种工业大麻品种（Białobrzeskie、Tygra、Henola 和 Rajan），以确定它们中哪一种是有效生产生物乙醇的最有利的原料。首先，对大麻生物质进行物理和化学预处理。研究发现，2%NaOH 的碱性处理最为有效，选择两种品种的生物质进行下一步研究：Tygra 和 Rajan。通过傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）分析预处理前后的大麻生物质，证实了预处理的有效性。接下来，使用响应面法对先前选择的参数进行酶解。然后，分析了两种方法：分别水解和发酵（SHF）和同步糖化和发酵（SSF）过程。在 SHF 过程中，Tygra 生物质的乙醇浓度为 10.5 g·L（3.04 m·ha），而在 SSF 过程中，Rajan 生物质的乙醇浓度为 7.5 g·L（2.23 m·ha）。总之，波兰大麻品种的生物质，即 Tygra 和 Rajan，被发现是生物乙醇生产的一种有趣且有前途的原料。
