Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, USA.
Department of Food Nutrition Dietetics & Health, Kansas State University, Manhattan, KS 66506, USA.
Bioresour Technol. 2020 Aug;309:123383. doi: 10.1016/j.biortech.2020.123383. Epub 2020 Apr 17.
In this work, four varieties of hemp biomasses (Helena, SS Beta, Tygra, and Elleta Campana) pretreated with liquid hot water (LHW), HSO, and NaOH were investigated for ethanol production. Physicochemical and morphological properties of the pretreated hemp biomass were characterized. LHW achieved high glucan (85-98%) and xylan (67-71%) recoveries. HSO induced significant glucan decomposition (5.9-10.6 g/L) and inhibitor formation (4.5-7.4 g/L of HMF and 2.8-4.5 g/L of furfural) in resulting slurries. Both LHW and HSO pretreatments resulted in low glucose and ethanol yields due to recondensed lignin units. NaOH pretreatment achieved high glucose and ethanol yields due to efficient lignin removal (58.6-75.3%). There was no significant variation in ethanol yield among the four hemp varieties pretreated by NaOH. HSO and NaOH pretreated biomasses showed apparent terraced-field structures and microporous protuberances. Changes in crystallinity indexes and intensities of FTIR peaks were consistent with enhanced cellulose and decreased amorphous hemicellulose.
本工作研究了经液态热水（LHW）、HSO 和 NaOH 预处理的四种大麻生物质（Helena、SS Beta、Tygra 和 Elleta Campana）用于乙醇生产。对预处理大麻生物质的物理化学和形态特性进行了表征。LHW 实现了高达 85-98%的葡聚糖和 67-71%的木聚糖回收率。HSO 在所得浆料中诱导了显著的葡聚糖分解（5.9-10.6 g/L）和抑制剂形成（4.5-7.4 g/L 的 HMF 和 2.8-4.5 g/L 的糠醛）。由于重新缩合的木质素单元，LHW 和 HSO 预处理均导致葡萄糖和乙醇产率较低。由于高效的木质素去除（58.6-75.3%），NaOH 预处理实现了高的葡萄糖和乙醇产率。NaOH 预处理的四种大麻生物质的乙醇产率没有显著差异。HSO 和 NaOH 预处理的生物质表现出明显的梯田结构和微孔突起。结晶度指数和 FTIR 峰强度的变化与纤维素的增强和无定形半纤维素的减少一致。
