Wang Jun, Conti David V, Epeldegui Marta, Ollikainen Miina, Tyndale Rachel F, Hwang Amie Eunah, Magpantay Larry, Mack Thomas McCulloch, Martinez-Maza Otoniel, Kaprio Jaakko, Cozen Wendy
Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA.
Center for Genetic Epidemiology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA.
Cancers (Basel). 2021 Oct 27;13(21):5395. doi: 10.3390/cancers13215395.
Smoking is associated with a moderate increased risk of Hodgkin and follicular lymphoma. To understand why, we examined lymphoma-related biomarker levels among 134 smoking and non-smoking twins (67 pairs) ascertained from the Finnish Twin Cohort. Previously collected frozen serum samples were tested for cotinine to validate self-reported smoking history. In total, 27 immune biomarkers were assayed using the Luminex Multiplex platform (R & D Systems). Current and non-current smokers were defined by a serum cotinine concentration of >3.08 ng/mL and ≤3.08 ng/mL, respectively. Associations between biomarkers and smoking were assessed using linear mixed models to estimate beta coefficients and standard errors, adjusting for age, sex and twin pair as a random effect. There were 55 never smokers, 43 current smokers and 36 former smokers. CCL17/TARC, sgp130, haptoglobin, B-cell activating factor (BAFF) and monocyte chemoattractant protein-1 (MCP1) were significantly ( < 0.05) associated with current smoking and correlated with increasing cotinine concentrations ( < 0.05). The strongest association was observed for CCL17/TARC ( = 0.0001). Immune biomarker levels were similar in former and never smokers. Current smoking is associated with increased levels of lymphoma-associated biomarkers, suggesting a possible mechanism for the link between smoking and risk of these two B-cell lymphomas.
吸烟与霍奇金淋巴瘤和滤泡性淋巴瘤风险适度增加有关。为了解其中原因，我们在从芬兰双胞胎队列中确定的134对吸烟和不吸烟双胞胎（67对）中检测了淋巴瘤相关生物标志物水平。对先前收集的冷冻血清样本进行可替宁检测，以验证自我报告的吸烟史。总共使用Luminex多重平台（R&D系统）检测了27种免疫生物标志物。当前吸烟者和非当前吸烟者分别由血清可替宁浓度>3.08 ng/mL和≤3.08 ng/mL定义。使用线性混合模型评估生物标志物与吸烟之间的关联，以估计β系数和标准误差，并将年龄、性别和双胞胎对作为随机效应进行调整。有55名从不吸烟者、43名当前吸烟者和36名既往吸烟者。CCL17/TARC、可溶性gp130、触珠蛋白、B细胞活化因子（BAFF）和单核细胞趋化蛋白-1（MCP1）与当前吸烟显著相关（<0.05），并与可替宁浓度升高相关（<0.05）。CCL17/TARC的关联最为显著（=0.0001）。既往吸烟者和从不吸烟者的免疫生物标志物水平相似。当前吸烟与淋巴瘤相关生物标志物水平升高有关，提示吸烟与这两种B细胞淋巴瘤风险之间联系的一种可能机制。
