The Affiliated People's Hospital, Ningbo University, Ningbo, China.
Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.
Int J Clin Oncol. 2022 Feb;27(2):354-361. doi: 10.1007/s10147-021-02073-w. Epub 2021 Nov 13.
Recent studies have shown that circular RNAs (circRNAs) are closely related to the occurrence and development of gastric cancer. In this paper, we analyzed the value of hsa_circ_0003195 in diagnosis and prognosis of gastric cancer.
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to determine the level of hsa_circ_0003195 in 100 paired gastric cancer tissues and paracancerous tissues, 74 paired fasting plasma from gastric cancer patients before and 10 days after operation, and 74 fasting plasmas from healthy controls. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic value. The survival analysis and Cox proportional-hazards model were used to evaluate the efficiency of hsa_circ_0003195 in predicting overall survival (OS) in patients with gastric cancer.
The expression of hsa_circ_0003195 was down-regulated in gastric cancer tissues and plasma from patients with gastric cancer. The expression level of hsa_circ_0003195 was correlated with differentiation, TNM stages, lymphatic metastasis, and distal metastasis. The area under the ROC curve (AUC) of tissue and plasma hsa_circ_0003195 was 0.684 and 0.695, respectively. The plasma hsa_circ_0003195 can be used as predictors of survival of patients with gastric cancer.
Hsa_circ_0003195 may be a new diagnostic and prognostic marker of gastric cancer.
最近的研究表明，环状 RNA（circRNAs）与胃癌的发生和发展密切相关。在本文中，我们分析了 hsa_circ_0003195 在胃癌诊断和预后中的价值。
采用定量逆转录聚合酶链反应（qRT-PCR）测定 100 对胃癌组织和癌旁组织、74 对胃癌患者手术前后空腹血浆和 74 例健康对照者的 hsa_circ_0003195 水平。绘制受试者工作特征（ROC）曲线评估诊断价值。生存分析和 Cox 比例风险模型用于评估 hsa_circ_0003195 预测胃癌患者总生存期（OS）的效率。
hsa_circ_0003195 在胃癌组织和胃癌患者血浆中表达下调。hsa_circ_0003195 的表达水平与分化、TNM 分期、淋巴转移和远处转移有关。组织和血浆 hsa_circ_0003195 的 ROC 曲线下面积（AUC）分别为 0.684 和 0.695。血浆 hsa_circ_0003195 可作为胃癌患者生存的预测因子。
hsa_circ_0003195 可能是胃癌的一种新的诊断和预后标志物。
