Food Engineering Department, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
Yeniçağa Yaşar Çelik Vocational School, Bolu Abant İzzet Baysal University, Bolu, Turkey.
Food Environ Virol. 2022 Mar;14(1):1-9. doi: 10.1007/s12560-021-09501-0. Epub 2021 Nov 13.
Salmonella contamination is a critical problem in poultry farms, with serious consequences for both animals and food products. The aim of this study is to investigate the use of phage cocktails to reduce Salmonella contamination in poultry farms. Within the scope of the study, Salmonella phages were isolated from chicken stool. After the host range of phages was determined, morphological characterization was performed through transmission electron microscopy analysis. Then, replication parameters and adsorption rates were determined by one-step growth curves. After that, phage cocktail was prepared, and its effectiveness was tested in three environments, which were drinking water, shavings, and plastic surfaces. The results obtained have demonstrated that the phage cocktail can reduce Salmonella count up to 2.80 log units in drinking water, up to 2.30 log units on shavings, and 2.31 log units on plastic surfaces. It has been determined that phage cocktails could be a successful alternative in reducing Salmonella contamination in poultry environment. This work is the first study to investigate the use of phage cocktails for reducing Salmonella contamination in poultry water and on shavings, and it is presumed that the results obtained will contribute to the fight against pathogens by making them applicable to poultry farms.
沙门氏菌污染是家禽养殖场的一个关键问题，对动物和食品产品都有严重的后果。本研究旨在探讨使用噬菌体鸡尾酒来减少家禽养殖场的沙门氏菌污染。在研究范围内，从鸡粪中分离出沙门氏菌噬菌体。在确定噬菌体的宿主范围后，通过透射电子显微镜分析进行形态特征描述。然后，通过一步生长曲线确定复制参数和吸附率。之后，制备了噬菌体鸡尾酒，并在三种环境中测试了其效果，这三种环境分别是饮用水、刨花和塑料表面。研究结果表明，噬菌体鸡尾酒可使饮用水中的沙门氏菌数量减少 2.80 个对数单位，使刨花中的沙门氏菌数量减少 2.30 个对数单位，使塑料表面上的沙门氏菌数量减少 2.31 个对数单位。噬菌体鸡尾酒可以作为减少家禽环境中沙门氏菌污染的一种成功替代方法。这项工作是首次研究使用噬菌体鸡尾酒来减少家禽饮用水和刨花中的沙门氏菌污染，预计所获得的结果将有助于通过将其应用于家禽养殖场来对抗病原体。
