多步富集工艺和定制生长培养基可提高从二级污水中氯胁迫大肠菌群的复苏率。
A multistep enrichment process with custom growth medium improves resuscitation of chlorine-stressed coliforms from secondary sewage effluents.
机构信息
Chemical and Biological Signatures Group, National Security Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA.
Statistical Modeling and Experiment Design Group, National Security Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA.
出版信息
J Microbiol Methods. 2022 Jan;192:106364. doi: 10.1016/j.mimet.2021.106364. Epub 2021 Nov 12.
Resuscitation and detection of stressed total coliforms in chlorinated water samples is needed to assess and prevent health effects from adverse exposure. In this study, we report that the addition of a growth enhancer mix consisting of trehalose, sodium pyruvate, magnesium chloride, and 1× trace mineral supplement improved growth of microorganisms from chlorinated secondary effluent in the base medium with Colilert-18. Improving growth of chlorine stressed microorganisms from secondary effluent is crucial to decreased detection time from 18 to 8 h.
为了评估和预防因不良接触而产生的健康影响，需要对氯消毒水样中的应激总大肠菌群进行复苏和检测。在这项研究中，我们报告称，在含有 Colilert-18 的基础培养基中添加海藻糖、丙酮酸钠、氯化镁和 1×痕量矿物质补充剂的生长增强混合液可改善氯化二级出水微生物的生长。改善二级出水中氯应激微生物的生长对于将检测时间从 18 小时缩短至 8 小时至关重要。
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