Lin S D
Appl Environ Microbiol. 1976 Oct;32(4):547-52. doi: 10.1128/aem.32.4.547-552.1976.
The standard one-step M-FC broth-membrane-filter procedure for recovery of fecal coliforms from chlorinated sewage effluents is much less effective than the multiple-tube (most-probable-number) technique. A two-step membrane-filter method, using a pre-enrichment technique with phenol red lactose broth and incubation at 35 degrees C for 4 h, followether 18+/-2 h, enhanced fecal coliform recovery from chlorinated effluents. The results of 126 comparisons using chlorinated effluents from five wastewater plants showed that fecal coliform recovery by using the two-step membrane-filter method is comparable to that using the multiple-tube procedure.
从经过氯化处理的污水中回收粪大肠菌群的标准单步M-FC肉汤-膜过滤法,其效果远不如多管(最可能数)技术。一种两步膜过滤法,采用酚红乳糖肉汤预富集技术,并在35℃下培养4小时,随后再培养18±2小时,可提高从经过氯化处理的污水中回收粪大肠菌群的效率。对来自五个污水处理厂的经过氯化处理的污水进行126次比较的结果表明,使用两步膜过滤法回收粪大肠菌群的效果与使用多管法相当。