全基因组鉴定揭示了 CEP 肽在黄瓜根发育中的功能。

Genome-wide identification reveals the function of CEP peptide in cucumber root development.

机构信息

Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, 330045, Nanchang, China.

Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, 330045, Nanchang, China; Key Laboratory of Ministry of Education for Crop Physiology, Ecology and Genetics and Breeding of Jiangxi Agricultural University, 330045 Nanchang, China.

出版信息

Plant Physiol Biochem. 2021 Dec;169:119-126. doi: 10.1016/j.plaphy.2021.11.007. Epub 2021 Nov 9.

DOI:10.1016/j.plaphy.2021.11.007

PMID:34775178

Abstract

C-Terminally Encoded (CEP) peptides are crucial plant growth regulators. Nevertheless, their physiological roles in cucumber (Cucumis sativus L.), an essential worldwide economical vegetable, remains untapped. In this study, 6 cucumber CEP (CsCEP) genes were identified. A comprehensive analysis showed that the CsCEP proteins displayed conserved characteristics to the identified CEP protein members in other species. CsCEP genes expression levels were variant in cucumber tissues, and were also differentially induced by several environmental factors, suggesting distinct and overlapping roles of CsCEPs in various cucumber developmental processes. We further revealed that synthetic CsCEP4 peptide promoted cucumber primary root growth in a reactive oxygen species (ROS) dependent manner. Overall, our work will provide fundamental insights into the crucial physiological roles of small bioactive peptides during cucumber root development.

摘要

C 端编码（CEP）肽是重要的植物生长调节剂。然而，它们在黄瓜（Cucumis sativus L.）中的生理作用——一种全球重要的经济蔬菜，尚未被开发。在这项研究中，鉴定了 6 个黄瓜 CEP（CsCEP）基因。综合分析表明，CsCEP 蛋白在其他物种中鉴定的 CEP 蛋白成员具有保守特征。CsCEP 基因在黄瓜组织中的表达水平存在差异，并且也被多种环境因素差异诱导，表明 CsCEPs 在各种黄瓜发育过程中具有不同但重叠的作用。我们进一步揭示了合成的 CsCEP4 肽以依赖活性氧（ROS）的方式促进黄瓜主根生长。总的来说，我们的工作将为小分子生物活性肽在黄瓜根发育过程中的关键生理作用提供基础见解。