Zhang Peipei, Gao Weidong, Guo Lijian, Chen Ming, Ma Jingfu, Tian Tian, Wang Yanjie, Zhang Xiwei, Wei Yongtong, Chen Tao, Yang Delong
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.
College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2024 Nov 25;25(23):12663. doi: 10.3390/ijms252312663.
The plant peptide-containing sulfated tyrosine (PSY) family plays critical roles in plant cell proliferation and stress responses. However, the functional characterization of the PSY peptide family in wheat remains unclear. This study systematically identified a total of 29 genes at the genome-wide level, classifying them into six subgroups based on PSY-like motifs. These peptides contain a highly conserved active peptide domain, closely resembling the AtPSY1 motif. All TaPSY homologs are predicted to have a sulfated tyrosine catalyzed by plant tyrosylprotein sulfotransferase (TPST). The genes displayed distinct expression patterns across various tissues, with most genes showing higher expression levels in roots and stems. Synthetic sulfated TaPSY peptides enhanced root growth in both wild-type and the mutant plants. In wheat, exogenous application of TaPSY peptides also promoted root growth, with the synthetic TaPSY5 peptide affecting reactive oxygen species levels in wheat taproots to stimulate primary root growth. Furthermore, transgenic plants overexpressing exhibited longer primary roots and increased lateral root numbers. These findings provide insights into the physiological roles of TaPSY peptides in regulating wheat root growth.
含植物肽的硫酸化酪氨酸（PSY）家族在植物细胞增殖和应激反应中起关键作用。然而，PSY肽家族在小麦中的功能特性仍不清楚。本研究在全基因组水平上系统鉴定了总共29个基因，根据类PSY基序将它们分为六个亚组。这些肽含有一个高度保守的活性肽结构域，与AtPSY1基序非常相似。所有TaPSY同源物预计都有一个由植物酪氨酸蛋白硫酸转移酶（TPST）催化的硫酸化酪氨酸。这些基因在不同组织中表现出不同的表达模式，大多数基因在根和茎中表达水平较高。合成的硫酸化TaPSY肽促进了野生型和突变体植物的根生长。在小麦中，外源施用TaPSY肽也促进了根生长，合成的TaPSY5肽影响小麦主根中的活性氧水平以刺激初生根生长。此外，过表达的转基因植物表现出更长的初生根和增加的侧根数。这些发现为TaPSY肽在调节小麦根生长中的生理作用提供了见解。
