Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin/College of Life Science, Tarim University, Alar, 843300, Xinjiang, People's Republic of China.
School of Basic Medicine, Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, People's Republic of China.
Antonie Van Leeuwenhoek. 2022 Jan;115(1):79-87. doi: 10.1007/s10482-021-01681-w. Epub 2021 Nov 13.
During a screening for novel and useful actinobacteria in desert animal, a new actinomycete was isolated and designated strain TRM63209. The strain was isolated from in vivo of a Blattella germanica in Tarim University in Alar City, Xinjiang, north-west China. The strain was found to exhibit an inhibitory effect on biofilm formation by Candida albicans ATCC 18,804. The strain was observed to form abundant aerial mycelium, occasionally twisted and which differentiated into spiral spore chains. Spores of TRM63209 were observed to be oval-shaped, with a smooth surface. Strain TRM63209 was found to grow optimally at 28 °C, pH 8 and in the presence of 1% (w/v) NaCl. The whole-cell sugars of strain TRM63209 were rhamnose ribose, xylose, mannose, galactose and glucose, and the principal polarlipids were found to be diphosphatidylglycerol, phos-phatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannoside, phosphatidylinositol and an unknown phospholipid(L). The diagnostic cell wall amino acid was identified as LL-diaminopimelic acid. The predominant menaquinone was found to be MK-9(H6) (14.64%), MK-9(H2) (19.65%), MK-9(H8) (22.34%), MK-10(H2) (25.37%). The major cellular fatty acids were identified as iso-C16:0, 16:0, anteiso-C15:0, anteiso-C17:0, iso-C15:0 and Sum in Feature 3. Analysis of the 16S rRNA sequence showed that strain TRM63209 exhibits high sequence similarity to Streptomyces bungoensis strain DSM 41781 98.20%. A multi-locus sequence analysis of five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) and phylogenomic analysis also illustrated that strain TRM63209 should be assigned to the genus Streptomyces. The DNA G + C content of the strain was determined to be 70.2 mol%. Average nucleotide identity (ANI) between strain TRM63209 and S. bungoensis DSM 41781, Streptomyces phyllanthi PA1-07, Streptomyces longwoodensis DSM 41677 and Streptomyces caeruleatus NRRL B-24802 were 82.76%, 82.54%, 82.65%, 84.02%, respectively. Digtal DNA-DNA (dDDH) hybridization were 26.30%, 25.10%, 26.20%, 29.50%, respectively. Therefore, it is concluded that strain TRM63209 represents a novel species of the genus Streptomyces, for which the name Streptomyces blattelae is proposed. The type strain is TRM63209 (CCTCC AA 2018093 = LMG 31,403 = TRM63209).
在对沙漠动物中新型有用放线菌的筛选过程中，分离到一株新型放线菌，命名为菌株 TRM63209。该菌株是从中国新疆阿拉尔塔里木大学的德国小蠊体内分离得到的。该菌株对白色念珠菌 ATCC 18804 的生物膜形成具有抑制作用。该菌株产生丰富的气生菌丝，偶尔扭曲并分化成螺旋孢子链。TRM63209 的孢子呈椭圆形，表面光滑。菌株 TRM63209 在 28°C、pH8 和 1%（w/v）NaCl 存在下生长最佳。菌株 TRM63209 的全细胞糖为鼠李糖、核糖、木糖、甘露糖、半乳糖和葡萄糖，主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰肌醇甘露糖苷、磷脂酰肌醇和一种未知的磷脂（L）。鉴定细胞壁氨基酸为 LL-二氨基庚二酸。主要的menaquinone 为 MK-9（H6）（14.64%）、MK-9（H2）（19.65%）、MK-9（H8）（22.34%）、MK-10（H2）（25.37%）。主要细胞脂肪酸为异-C16:0、16:0、anteiso-C15:0、anteiso-C17:0、iso-C15:0 和特征 3 中的总和。16S rRNA 序列分析表明，菌株 TRM63209 与链霉菌属 bungoensis 菌株 DSM 41781 的序列相似度高达 98.20%。对五个看家基因（atpD、gyrB、rpoB、recA 和 trpB）的多位点序列分析和系统基因组分析也表明，菌株 TRM63209 应归属于链霉菌属。该菌株的 DNA G+C 含量为 70.2mol%。菌株 TRM63209 与链霉菌属 bungoensis DSM 41781、菲律宾链霉菌 PA1-07、长枝链霉菌 DSM 41677 和蓝灰链霉菌 NRRL B-24802 的平均核苷酸同一性（ANI）分别为 82.76%、82.54%、82.65%和 84.02%。数字 DNA-DNA 杂交（dDDH）分别为 26.30%、25.10%、26.20%和 29.50%。因此，结论是菌株 TRM63209 代表链霉菌属的一个新型种，命名为拟诺卡氏菌 blattelae。模式菌株为 TRM63209（CCTCC AA 2018093=LMG 31403=TRM63209）。
