Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin/College of Life Science, Tarim University, Alar, 843300, Xinjiang, People's Republic of China.
Antonie Van Leeuwenhoek. 2020 Jul;113(7):1023-1031. doi: 10.1007/s10482-020-01416-3. Epub 2020 Apr 18.
A novel Streptomyces strain, designated TRM43335, was isolated from the Taklimakan desert in Alar City, Xinjiang, north-west China. The strain was found to exhibit an inhibitory effect on biofilm formation by Candida albicans and Staphylococcus epidermidis. A polyphasic approach was used to determine its taxonomic status. The strain was observed to form abundant aerial mycelium, occasionally twisted and which differentiated into spiral spore chains. Spores of TRM43335 were observed to be oval-shaped, with a smooth surface. Strain TRM43335 was found to grow optimally at 37 °C, pH 8 and in the presence of 1% (w/v) NaCl. The major sugars were identified as ribose, xylose, glucose, mannose and galactose, and the principal phospholipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The diagnostic cell wall amino acid was identified as LL-diaminopimelic acid. The predominant menaquinone was found to be MK-9(H). The major cellular fatty acids were identified as iso-C H, anteiso-C, iso-C, anteiso-C, anteiso-C w9c and iso-C. Analysis of the 16S rRNA sequence showed that strain TRM43335 exhibits high sequence similarity to Streptomyces desertarenae SYSU D8023 98.69%. A multilocus sequence analysis of five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) also illustrated that strain TRM43335 should be assigned to the genus Streptomyces. The DNA G + C content of the strain was determined to be 72.8 mol%. The average nucleotide identity relatedness between strain TRM43335 and the phylogenetically related strain S. desertarenae SYSU D8023 was found to be 89.23%, and the in silico DNA-DNA hybridization value to be 36.70%. Therefore, it is concluded that strain TRM43335 represents a novel species of the genus Streptomyces, for which the name Streptomyces taklimakanensis sp. nov is proposed. The type strain is TRM43335 (CCTCC AA 2018052 = KCTC 49254).
一株新型链霉菌菌株，被命名为 TRM43335，从中国西北部新疆阿拉尔市的塔克拉玛干沙漠中分离得到。该菌株被发现对白色念珠菌和表皮葡萄球菌的生物膜形成具有抑制作用。采用多相分类方法来确定其分类地位。观察到该菌株形成丰富的气生菌丝，偶尔扭曲并分化成螺旋孢子链。TRM43335 的孢子呈椭圆形，表面光滑。菌株 TRM43335 在 37°C、pH8 和 1%（w/v）NaCl 的条件下生长最佳。主要糖为核糖、木糖、葡萄糖、甘露糖和半乳糖，主要磷脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇甘露糖苷和磷脂酰肌醇。鉴定的细胞壁氨基酸为 LL-二氨基庚二酸。主要的menaquinone 为 MK-9(H)。主要的细胞脂肪酸为 iso-C H、anteiso-C、iso-C、anteiso-C、anteiso-C w9c 和 iso-C。16S rRNA 序列分析表明，菌株 TRM43335 与链霉菌荒漠亚种 SYSU D8023 的序列相似性高达 98.69%。对五个看家基因（atpD、gyrB、rpoB、recA 和 trpB）的多位序列分析也表明，菌株 TRM43335 应归属于链霉菌属。该菌株的 DNA G+C 含量为 72.8mol%。菌株 TRM43335 与系统发育上相关的链霉菌荒漠亚种 SYSU D8023 的平均核苷酸身份相关度为 89.23%，体外 DNA-DNA 杂交值为 36.70%。因此，我们认为菌株 TRM43335 代表了链霉菌属的一个新种，将其命名为塔克拉玛干链霉菌新种。其模式菌株为 TRM43335（CCTCC AA 2018052=KCTC 49254）。
