Shagieva Ekaterina, Demnerova Katerina, Michova Hana
Laboratory of Food Microbiology, Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Czechia.
Front Microbiol. 2021 Oct 27;12:730858. doi: 10.3389/fmicb.2021.730858. eCollection 2021.
is regarded as the leading cause of bacterial gastroenteritis around the world. Even though it is generally considered to be a sensitive microaerobic pathogen, it is able to survive in the environment outside of the intestinal tract of the host. This study aimed to assess the impact of selected environmental parameters on the survival of 14 isolates of different origins, including 12 water isolates. The isolates were tested for their antibiotic resistance, their ability to survive at low temperature (7°C), develop aerotolerance, and to interact with the potential protozoan host . The antibiotic susceptibility was determined by standard disk diffusion according to EUCAST. Out of the 14 isolates, 8 were resistant to ciprofloxacin (CIP) and 5 to tetracycline (TET), while only one isolate was resistant to erythromycin (ERY). Five isolates were resistant to two different antibiotic classes. Tetracycline resistance was only observed in isolates isolated from wastewater and a clinical sample. Further, the isolates were tested for their survival at 7°C under both aerobic and microaerobic conditions using standard culture methods. The results showed that under microaerobic conditions, all isolates maintained their cultivability for 4 weeks without a significant decrease in the numbers of bacteria and variation between the isolates. However, significant differences were observed under aerobic conditions (AC). The incubation led to a decrease in the number of cultivable cells, with complete loss of cultivability after 2 weeks (one water isolate), 3 weeks (7 isolates), or 4 weeks of incubation (6 isolates). Further, all isolates were studied for their ability to develop aerotolerance by repetitive subcultivation under microaerobic and subsequently AC. Surprisingly, all isolates were able to adapt and grow under AC. As the last step, 5 isolates were selected to evaluate a potential protective effect provided by . The cocultivation of isolates with the amoeba resulted in the survival of about 40% of cells treated with an otherwise lethal dose of gentamicin. In summary, is able to adapt and survive in a potentially detrimental environment for a prolonged period of time, which emphasizes the role of the environmental transmission route in the spread of campylobacteriosis.
被认为是全球细菌性肠胃炎的主要病因。尽管它通常被认为是一种敏感的微需氧病原体，但它能够在宿主肠道外的环境中存活。本研究旨在评估选定的环境参数对14株不同来源分离株存活的影响，其中包括12株水源性分离株。对这些分离株进行了抗生素耐药性测试、在低温（7°C）下存活的能力测试、耐氧性发展测试以及与潜在原生动物宿主相互作用的测试。根据欧洲抗菌药物敏感性试验委员会（EUCAST）的标准纸片扩散法测定抗生素敏感性。在这14株分离株中，8株对环丙沙星（CIP）耐药，5株对四环素（TET）耐药，而只有1株对红霉素（ERY）耐药。5株分离株对两种不同的抗生素类别耐药。四环素耐药性仅在从废水和一份临床样本中分离出的分离株中观察到。此外，使用标准培养方法在有氧和微需氧条件下对分离株在7°C下的存活情况进行了测试。结果表明，在微需氧条件下，所有分离株在4周内保持其可培养性，细菌数量没有显著减少，且分离株之间没有差异。然而，在有氧条件（AC）下观察到了显著差异。培养导致可培养细胞数量减少，在培养2周（1株水源性分离株）、3周（7株）或4周（6株）后可培养性完全丧失。此外，通过在微需氧和随后的有氧条件下重复传代培养，研究了所有分离株产生耐氧性的能力。令人惊讶的是，所有分离株都能够在有氧条件下适应并生长。作为最后一步，选择了5株分离株来评估[此处原文缺失相关内容]提供的潜在保护作用。分离株与变形虫的共培养导致约40%用否则致死剂量庆大霉素处理的细胞存活。总之，[此处原文缺失相关内容]能够在潜在有害环境中长时间适应并存活，这突出了环境传播途径在弯曲杆菌病传播中的作用。
