甘氨酰-L-谷氨酰胺对猫节前去神经支配的颈上神经节中乙酰胆碱酯酶和丁酰胆碱酯酶维持的直接神经营养作用。
Direct neurotrophic action of glycyl-L-glutamine in the maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat.
作者信息
Koelle G B, Sanville U J, Thampi N S
机构信息
Department of Pharmacology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6084.
出版信息
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6944-7. doi: 10.1073/pnas.84.19.6944.
Abstract
Intracarotid infusion of glycyl-L-glutamine (Gly-Gln) was shown previously to oppose the fall in the acetylcholinesterase and butyrylcholinesterase contents of the cat superior cervical ganglion (SCG) that otherwise follows preganglionic denervation. However, its effect was demonstrable only on the vascularly remote left SCG but not on the directly infused right SCG. Accordingly, it was concluded that a metabolite of Gly-Gln, formed in the blood, is an active neurotrophic factor. Glycyl-L-glutamic acid and L-glutamic acid were subsequently found to have a similar but less marked effect on both SCG. In the present study an alternative explanation has been tested: that Gly-Gln must combine slowly with some component of plasma to enable it to penetrate the ganglion cells and exert its neurotrophic effect. Findings are consistent with the latter proposal.
摘要
先前的研究表明，颈内动脉注入甘氨酰-L-谷氨酰胺（Gly-Gln）可对抗猫颈上神经节（SCG）中乙酰胆碱酯酶和丁酰胆碱酯酶含量的下降，否则这种下降会在节前神经去神经支配后出现。然而，其作用仅在血管较远的左侧SCG上得到证实，而在直接注入的右侧SCG上未得到证实。因此，得出的结论是，在血液中形成的Gly-Gln代谢产物是一种活性神经营养因子。随后发现甘氨酰-L-谷氨酸和L-谷氨酸对两个SCG都有类似但不太明显的作用。在本研究中，对另一种解释进行了验证：Gly-Gln必须与血浆中的某些成分缓慢结合，使其能够穿透神经节细胞并发挥其神经营养作用。研究结果与后一种观点一致。
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Direct neurotrophic action of glycyl-L-glutamine in the maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat.甘氨酰-L-谷氨酰胺对猫节前去神经支配的颈上神经节中乙酰胆碱酯酶和丁酰胆碱酯酶维持的直接神经营养作用。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A new and rapid colorimetric determination of acetylcholinesterase activity.一种新的快速比色法测定乙酰胆碱酯酶活性。
Biochem Pharmacol. 1961 Jul;7:88-95. doi: 10.1016/0006-2952(61)90145-9.
3
Electron microscope localization of acetylcholinesterase and butyrylcholinesterase in the superior cervical ganglion of the cat. II. Preganglionically denervated ganglion.猫颈上神经节中乙酰胆碱酯酶和丁酰胆碱酯酶的电子显微镜定位。II. 节前神经切断的神经节
J Cell Biol. 1981 Mar;88(3):581-90. doi: 10.1083/jcb.88.3.581.
4
Regeneration of cholinesterases in the stellate and normal and denervated superior cervical ganglion of the cat following inactivation by sarin.猫的星状神经节以及正常和去神经支配的颈上神经节中胆碱酯酶在被沙林灭活后的再生情况。
J Neurochem. 1982 Jun;38(6):1695-8. doi: 10.1111/j.1471-4159.1982.tb06651.x.
5
Glycyl glutamine, an inhibitory neuropeptide derived from beta-endorphin.甘氨酰谷氨酰胺，一种源自β-内啡肽的抑制性神经肽。
Nature. 1983;306(5940):267-70. doi: 10.1038/306267a0.
6
Demonstration of a neurotrophic factor for the maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat.猫颈上神经节节前去神经支配后维持乙酰胆碱酯酶和丁酰胆碱酯酶的神经营养因子的证明。
Proc Natl Acad Sci U S A. 1983 May;80(10):3106-10. doi: 10.1073/pnas.80.10.3106.
7
Partial characterization of the neurotrophic factor for maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat in vivo.体内猫颈上神经节节前去神经支配后维持乙酰胆碱酯酶和丁酰胆碱酯酶的神经营养因子的部分特性
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6539-42. doi: 10.1073/pnas.81.20.6539.
8
Studies on free amino acid metabolism in excised cervical sympathetic ganglia from the rat.大鼠离体颈交感神经节中游离氨基酸代谢的研究。
J Neurochem. 1966 Dec;13(12):1421-31. doi: 10.1111/j.1471-4159.1966.tb04303.x.
9
[Carboxypeptidases (kininases) from human and rabbit serum--isolation, purification and properties].[来自人及兔血清的羧肽酶（激肽酶）——分离、纯化及性质]
Biokhimiia. 1968 Sep-Oct;33(5):1012-22.
10
Detection of a lysosomal carboxypeptidase and a lysosomal dipeptidase in highly-purified dipeptidyl aminopeptidase I (cathepsin C) and the elimination of their activities from preparations used to sequence peptides.在高度纯化的二肽基肽酶I（组织蛋白酶C）中检测到一种溶酶体羧肽酶和一种溶酶体二肽酶，并从用于肽测序的制剂中去除它们的活性。
Biochem Biophys Res Commun. 1972 Jan 14;46(1):62-70. doi: 10.1016/0006-291x(72)90630-4.
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