Koelle G B, Ruch G A
Proc Natl Acad Sci U S A. 1983 May;80(10):3106-10. doi: 10.1073/pnas.80.10.3106.
Under sodium pentobarbital anesthesia, the superior cervical ganglia of cats were preganglionically denervated bilaterally. The following day cats were reanesthetized, the external carotid and lingual arteries were ligated bilaterally, and the right common carotid artery was infused for 24 hr with an extract prepared from cat brain, spinal cord, and sciatic nerves, with and without the incorporation of aprotinin, an inhibitor of proteases. They were sacrificed 48 hr after denervation, and the acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) and butyrylcholinesterase (acylcholine acylhydrolase, EC 3.1.1.8) contents of the superior cervical ganglia were compared with those of similarly denervated control ganglia. Both types of extract produced a significant reduction in the loss of both enzymes from the superior cervical ganglia, as did infusions of aprotinin alone. These findings demonstrate the presence of an endogenous neurotrophic factor for the maintenance of ganglionic acetylcholinesterase and butyrylcholinesterase. Its possible mechanisms of action, and those of aprotinin, are discussed.
在戊巴比妥钠麻醉下，对猫双侧颈上神经节进行节前去神经支配。次日，再次麻醉猫，双侧结扎颈外动脉和舌动脉，并分别向右侧颈总动脉灌注含有和不含有蛋白酶抑制剂抑肽酶的由猫脑、脊髓和坐骨神经制备的提取物24小时。去神经支配48小时后处死动物，比较颈上神经节中乙酰胆碱酯酶（乙酰胆碱乙酰水解酶，EC 3.1.1.7）和丁酰胆碱酯酶（酰基胆碱酰基水解酶，EC 3.1.1.8）的含量与同样去神经支配的对照神经节的含量。两种提取物以及单独灌注抑肽酶均使颈上神经节中两种酶的损失显著减少。这些发现证明存在一种内源性神经营养因子以维持神经节中的乙酰胆碱酯酶和丁酰胆碱酯酶。文中讨论了其可能的作用机制以及抑肽酶的作用机制。
