Latt Khun Zaw, Heymann Jurgen, Yoshida Teruhiko, Kopp Jeffrey B
Kidney Disease Section, Kidney Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, United States.
Front Med (Lausanne). 2021 Oct 29;8:761996. doi: 10.3389/fmed.2021.761996. eCollection 2021.
Recent advances in single-cell technology have enabled investigation of genomic profiles and molecular crosstalk among individual cells obtained from tissues and biofluids at unprecedented resolution. Glomerular diseases, either primary or secondary to systemic diseases, often manifest elements of inflammation and of innate and adaptive immune responses. Application of single-cell methods have revealed cellular signatures of inflammation, cellular injury, and fibrosis. From these signatures, potential therapeutic targets can be inferred and in theory, this approach might facilitate identification of precision therapeutics for these diseases. Single-cell analyses of urine samples and skin lesions from patients with lupus nephritis and of urine samples from patients with diabetic nephropathy and focal segmental glomerulosclerosis have presented potential novel approaches for the diagnosis and monitoring of disease activity. These single-cell approaches, in contrast to kidney biopsy, are non-invasive and could be repeated multiple times as needed.
单细胞技术的最新进展使得能够以前所未有的分辨率研究从组织和生物流体中获取的单个细胞之间的基因组图谱和分子相互作用。肾小球疾病，无论是原发性的还是继发于全身性疾病的，通常都表现出炎症以及先天性和适应性免疫反应的特征。单细胞方法的应用揭示了炎症、细胞损伤和纤维化的细胞特征。从这些特征中，可以推断出潜在的治疗靶点，理论上，这种方法可能有助于识别这些疾病的精准治疗方法。对狼疮性肾炎患者的尿液样本和皮肤病变以及糖尿病肾病和局灶节段性肾小球硬化患者的尿液样本进行单细胞分析，为疾病活动的诊断和监测提供了潜在的新方法。与肾活检相比，这些单细胞方法是非侵入性的，可以根据需要多次重复。
