School of Chemistry, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, UK.
Org Biomol Chem. 2021 Dec 1;19(46):10081-10084. doi: 10.1039/d1ob01898a.
Genome homology and the presence of a putative biosynthetic gene cluster identified DSM 41692 and DSM 41465 as candidate producers of the antibiotic nucleocidin 1. Indeed when these bacterial strains were cultured in a medium supplemented with fluoride (4 mM) they each produced nucleocidin 1 and the previously identified 4'-fluoro-3'--β-glucosylated adenosine 2 and its sulfamylated derivative 3. In both of these cases 4'-fluoroadenosine 9 is also identified as a natural product although it has never been observed during fermentations of , the original source of nucleocidin 1. The identity of 4'-fluoroadenosine 9 was confirmed by a total synthesis as well as by its enzymatic conversion to metabolite 2 using the glucosyl transferase enzyme, NucGT.
基因组同源性和假定生物合成基因簇的存在将 DSM 41692 和 DSM 41465 鉴定为抗生素核菌素 1 的候选产生菌。事实上,当这些细菌菌株在含有氟化物(4 mM)的培养基中培养时,它们都产生了核菌素 1 以及先前鉴定的 4'-氟-3'--β-葡萄糖基腺苷 2 和其磺酰胺化衍生物 3。在这两种情况下,4'-氟腺苷 9 也被鉴定为天然产物,尽管在核菌素 1 的原始来源 的发酵过程中从未观察到过。4'-氟腺苷 9 的身份通过全合成以及使用葡糖基转移酶 NucGT 将其转化为代谢物 2 的酶促转化得到了确认。