在英国户外环境中建立三角褐指藻生物炼制厂。
Towards a Phaeodactylum tricornutum biorefinery in an outdoor UK environment.
机构信息
Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield S1 3JD, UK.
Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield S1 3JD, UK.
出版信息
Bioresour Technol. 2022 Jan;344(Pt B):126320. doi: 10.1016/j.biortech.2021.126320. Epub 2021 Nov 13.
A series of commercial powdered media (Cell-Hi F2P, JWP and WP) and a hydroponics medium (FloraMicroBloom) were investigated for the cultivation of P. tricornutum, and compared with f/2 (a commonly employed laboratory cultivation medium; costlier to scale). Cell-Hi JWP showed good performance characteristics including cost-effectiveness. Outdoor cultivation of P. tricornutum in an airlift photobioreactor, using Cell-Hi JWP in the United Kingdom (UK) during September and October (average daily temperature ranging between 8 and 18 °C and natural sunlight) was comparable to cultivation indoors under controlled temperature and lighting. A strong positive correlation between fucoxanthin and chlorophyll a content, and a weak inverse correlation between eicosapentaenoic (EPA) content and temperature were observed. Commensal bacterial counts revealed a sinusoidal growth profile with a change in community dominance from Halomonas sp. to Marinobacter sp. This investigation reveals for the first time that a multi-product approach can be adopted with P. tricornutum in a UK outdoor environment using commercially viable powdered media.
研究了一系列商业粉末培养基（Cell-Hi F2P、JWP 和 WP）和一种水培培养基（FloraMicroBloom），用于培养 P. tricornutum，并与 f/2（一种常用的实验室培养培养基；规模扩大成本更高）进行了比较。Cell-Hi JWP 表现出良好的性能特点，包括成本效益。在气升式光生物反应器中，使用 Cell-Hi JWP 在英国（UK）进行了 P. tricornutum 的户外培养，在 9 月和 10 月（平均日温在 8 到 18°C 之间，自然阳光），与在受控温度和光照条件下的室内培养相当。观察到叶黄素和叶绿素 a 含量之间存在强烈的正相关，二十碳五烯酸（EPA）含量与温度之间存在较弱的负相关。共生细菌计数显示出正弦增长曲线，群落优势从 Halomonas sp. 变为 Marinobacter sp. 这一研究首次表明，在英国户外环境中，使用商业可行的粉末培养基，可以采用多产品方法来培养 P. tricornutum。
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