Showe L C, Ballantine M, Huebner K
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Genomics. 1987 Sep;1(1):71-6. doi: 10.1016/0888-7543(87)90107-8.
We have isolated genomic DNA clones which code for the human erythroid membrane protein band 3 (EMPB3). The identification of the gene has been confirmed by comparison of the amino acid sequence derived from the nucleotide sequence for two restriction fragments from the 5' end of the gene. Two exons have been identified. One exon encodes 20 amino acids which are identical to residues 36 to 56 of the band 3 protein, and the other encodes 44 amino acids homologous to residues 118 to 162. Southern analysis of genomic DNA derived from a panel of rodent-human somatic cell hybrids, which retain different complements of human chromosomes, with band 3 probes has allowed us to localize EMPB3 to human chromosome 17. The gene has been further localized between 17q21 and qter by analysis of DNA from somatic cell hybrids which carry derivative chromosomes from translocations involving chromosome 17 and either chromosome 15 or 21.
我们已经分离出编码人类红细胞膜蛋白带3（EMPB3）的基因组DNA克隆。通过比较从该基因5'端的两个限制性片段的核苷酸序列推导的氨基酸序列，已证实了该基因的鉴定。已鉴定出两个外显子。一个外显子编码20个氨基酸，与带3蛋白的36至56位残基相同，另一个外显子编码44个氨基酸，与118至162位残基同源。用带3探针分析来自保留不同人类染色体互补的一组啮齿动物 - 人类体细胞杂种的基因组DNA，使我们能够将EMPB3定位到人类染色体17上。通过分析来自携带涉及染色体17与染色体15或21的易位产生的衍生染色体的体细胞杂种的DNA，该基因已进一步定位在17q21和qter之间。
