Vassiliadis P, Mavrommati C, Kalapothaki V, Chronas G, Efstratiou M
J Hyg (Lond). 1985 Aug;95(1):139-47. doi: 10.1017/s0022172400062379.
A total of 574 samples, of seven different types, were examined for the presence of salmonellas. All the specimens were pre-enriched in buffered peptone water and enriched in Rappaport-Vassiliadis medium (RV medium). In one trial 0.1 ml of pre-enrichment culture of 497 samples (79 chicken carcasses, 228 specimens of minced meat, 100 pork sausages, 19 samples of dried powdered chicken meat, 11 specimens of faeces of healthy pigs and 60 samples of sewage polluted natural sea water) was seeded to 10 ml as well as to 100 ml of RV medium. With the first inoculum (ratio 0.1:10), 111 samples were found to contain salmonellas, while with the second inoculum (ratio 0.1:100), only 102 positive samples were detected. This difference is marginally significant (P less than 0.05). In another trial, 0.1 ml, 0.2 ml and 0.5 ml of pre-enrichment culture of 162 specimens (71 chicken carcasses, 40 samples of sewage polluted sea water and 51 samples of sewage polluted river water) were in turn introduced to 10 ml of RV medium. With the 0.1 ml inoculum 93 positive samples were detected, while with the 0.2 and 0.5 ml inocula 93 and 88 positive samples were found. The differences are not statistically significant. In these trials the growth of competing organisms was minimal with ratios of inocula 0.1:10 or 0.1:100.
共检测了574份7种不同类型的样本，以确定其中是否存在沙门氏菌。所有样本均先在缓冲蛋白胨水中进行预富集，然后在Rappaport-Vassiliadis培养基（RV培养基）中进行富集。在一项试验中，将497份样本（79份鸡 carcasses、228份碎肉样本、100份猪肉香肠、19份鸡肉干粉样本、11份健康猪粪便样本和60份受污水污染的天然海水样本）的预富集培养物0.1毫升分别接种到10毫升和100毫升的RV培养基中。对于第一次接种（比例0.1:10），发现111份样本含有沙门氏菌，而对于第二次接种（比例0.1:100），仅检测到102份阳性样本。这种差异具有边际显著性（P小于0.05）。在另一项试验中，将162份样本（71份鸡 carcasses、40份受污水污染的海水样本和51份受污水污染的河水样本）的预富集培养物0.1毫升、0.2毫升和0.5毫升依次接种到10毫升的RV培养基中。对于0.1毫升接种量，检测到93份阳性样本，而对于0.2毫升和0.5毫升接种量，分别发现93份和88份阳性样本。这些差异无统计学意义。在这些试验中，接种比例为0.1:10或0.1:100时，竞争菌的生长极少。 （注：原文中“chicken carcasses”未给出准确中文释义，暂保留英文）
