用于从海鸥泄殖腔拭子中分离沙门氏菌的富集培养基的比较。
A comparison of enrichment media for the isolation of salmonellae from seagull cloacal swabs.
作者信息
Fricker C R, Girdwood R W, Munro D
出版信息
J Hyg (Lond). 1983 Aug;91(1):53-8. doi: 10.1017/s0022172400060022.
Abstract
The relative efficiency of three selective enrichment broths (Muller-Kauffmann tetrathionate. Rappaport's and selenite F) was investigated for the isolation of salmonellae from seagull cloacal swabs. Pre-enrichment in buffered peptone water was employed throughout the study, which involved the examination of 560 gulls, sixty (10.7%) of which were found to be carrying salmonellae. Rappaport's broth as modified by Vassiliadis for incubation at 43 degrees C (Vassiliadis et al. 1976) yielded the highest number of positive swabs (57) and the widest range of serotypes. It was significantly more efficient that either selenite F or tetrathionate broth, although the results obtained with Rappaport's broth incubated at 37 and 43 degrees C did not differ significantly (P greater than 0.5). Eleven serotypes were isolated during the study, the most prevalent being Salmonella virchow.
摘要
研究了三种选择性增菌肉汤（穆勒 - 考夫曼四硫磺酸盐肉汤、拉帕波特肉汤和亚硒酸盐F肉汤）从海鸥泄殖腔拭子中分离沙门氏菌的相对效率。在整个研究中均采用缓冲蛋白胨水进行预增菌，该研究共检查了560只海鸥，其中60只（10.7%）被发现携带沙门氏菌。经瓦西利亚迪斯改良用于在43℃培养的拉帕波特肉汤（瓦西利亚迪斯等人，1976年）产生的阳性拭子数量最多（57个），血清型范围最广。它比亚硒酸盐F肉汤或四硫磺酸盐肉汤的效率显著更高，尽管在37℃和43℃培养的拉帕波特肉汤所获得的结果差异不显著（P大于0.5）。在研究期间分离出11种血清型，最常见的是猪霍乱沙门氏菌。
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