使用两亲超分子组装体进行多通道双蛋白传感。
Multichannel dual protein sensing using amphiphilic supramolecular assemblies.
机构信息
Department of Chemistry, University of Massachusetts Amherst, MA, 01002, USA.
出版信息
Chem Commun (Camb). 2021 Nov 30;57(95):12828-12831. doi: 10.1039/d1cc05407d.
Abstract
Protein sensing strategies have implications in detection of many human pathologies. Here, a supramolecular strategy for sensing two different proteins using a multichannel readout approach is outlined. Protein-ligand binding or enzymatic cleavage can both be programmed to induce supramolecular disassembly, which leads to fluorescence enhancement aggregation-induced emission (AIE), protein-induced fluorescence enhancement (PIFE), or disassembly-induced fluorescence enhancement (DIFE). The accompanying signal change from two different fluorophores and their patterns are then used for specific protein sensing.
摘要
蛋白质传感策略在许多人类病理的检测中具有重要意义。在这里，概述了一种使用多通道读出方法来感应两种不同蛋白质的超分子策略。蛋白质-配体结合或酶切都可以被编程以诱导超分子解体，这导致荧光增强聚集诱导发射（AIE）、蛋白质诱导荧光增强（PIFE）或解体诱导荧光增强（DIFE）。然后，使用来自两个不同荧光团的伴随信号变化及其模式来进行特定的蛋白质传感。
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